德州驴与泌阳驴睾丸组织mRNA与microRNA表达鉴定与分析  

Identification and Analysis of mRNA and microRNA Expression of Testis Tissue in Dezhou and Biyang Donkeys

作　　者：韩浩园 李婉涛 雷初朝[2] HAN Haoyuan;LI Wantao;LEI Chuzhao(College of Animal Science and Technology,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China;Henan Genetic Protection Engineering Research Center for Livestock and Poultry,Zhengzhou 450046,China)

机构地区：[1]河南牧业经济学院动物科技学院,郑州450046 [2]西北农林科技大学动物科技学院,杨凌712100 [3]河南省畜禽遗传保护工程技术研究中心,郑州450046

出　　处：《中国畜牧兽医》2019年第8期2371-2380,共10页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(81270439)

摘　　要：试验旨在探究驴睾丸组织中mRNA和microRNA的表达模式,为中国地方驴的转录组研究提供遗传数据。利用RNA-Seq和生物信息学方法分析了中国地方驴睾丸组织表达基因和microRNA,并分析了泌阳驴和德州驴品种间差异表达基因及microRNA。结果表明,mRNA平均clean reads为57 837 506条,Small RNA平均clean reads为9 952 015条,分别占原始数据的96.34%和88.86%。在驴睾丸组织中共发现24 751个表达基因及1 074个表达microRNAs,其中,睾丸组织表达量较高的基因为LOC 106836782、XLOC_066401、HSP90AA1、TNP 1和COF 2,表达量最高的microRNAs为eca-miR-143、eca-miR-21、eca-miR-99a、eca-miR-148a和eca-miR-26a。对泌阳驴与德州驴睾丸组织进行差异表达分析共发现102个差异表达基因及2个差异表达microRNAs,但未富集到功能条目,说明泌阳驴和德州驴品种间差异不明显。本研究提供了驴睾丸组织mRNA及microRNA表达模式和转录组学数据,并鉴定了泌阳驴与德州驴睾丸组织的差异表达mRNA和microRNA,对驴的遗传资源研究具有一定的科学意义。This study was aimed to explore the expression patterns of mRNA and microRNA in donkey testis,and provide genetic data for transcriptome studies of Chinese local donkeys.The mRNA and microRNA expressed in testis of Chinese domestic donkeys were analyzed by RNA-Seq and bioinformatics methods,and the differentially expressed genes and microRNA between Biyang and Dezhou donkeys were identified.The results showed that 57 837 506(accounted for 96.34%)and 9 952 015(accounted for 88.86%)clean reads of mRNA and Small RNA were obtained.A total of 24 751 genes and 1 074 microRNAs expressed in testis were identified.The top five expressed genes were LOC 106836782,XLOC_066401,HSP90AA1,TNP 1 and COF 2,the top five expressed microRNAs were eca-miR-143,eca-miR-21,eca-miR-99a,eca-miR-148a and eca-miR-26a.The results revealed 102 differentially expressed genes and 2 microRNAs between Biyang and Dezhou donkeys.However,no significant function term was annotated,which suggested that there was no obvious difference of genetic expression pattern between Biyang and Dezhou donkeys.This study provided gene expression pattern and transcriptome data for donkey testis tissue and identified differentially expressed mRNA and microRNA between Biyang and Dezhou donkeys,which had scientific significance for studying genetic sources of donkeys in the future.

关 键 词：驴 睾丸 转录组 差异表达基因 MICRORNA 

分 类 号：S822[农业科学—畜牧学]