雷奈酸锶对抗地塞米松诱导大鼠骨质疏松的作用  

Effect of strontium ranelate on dexamethasone-induced osteoporosis in rats

作　　者：黄歆[1] 汪瑜[1] 罗梅懿 宋永丹 杨浩森[1] HUANG Xin;WANG Yu;LUO Meiyi;SONG Yongdan;YANG Haosen(Department of Orthopaedics,Chengdu Seventh People’s Hospital,Chengdu,Sichuan 610000,China;Department of Orthopaedics,Sichuan Provincial People’s Hospital,Chengdu,Sichuan 610000,China)

机构地区：[1]成都市第七人民医院骨科,四川成都610000 [2]四川省人民医院骨科,四川成都610000

出　　处：《安徽医药》2019年第9期1724-1728,I0002,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究雷奈酸锶对地塞米松诱导骨质疏松大鼠的作用。方法30只雌性SD大鼠采用随机数字表法分为5组:空白组,骨质疏松模型组,雷奈酸锶高、中、低剂量组。5周后进行骨形态学及血清指标检测,同时考察不同浓度雷奈酸锶对成骨细胞的体外增殖影响。结果较模型组(38.00±2.36)μm,雷奈酸锶中、高剂量组的平均骨小梁厚度(48.50±4.05)μm、(52.00±4.44)μm明显升高(P<0.05),较模型组(189.90±19.27)μm,雷奈酸锶中、高剂量组的骨小梁间距(154.26±13.86)μm、(150.02±20.86)μm明显降低(P<0.05),表明雷奈酸锶的干预能明显改善骨显微结构指标。雷奈酸锶组高剂量较模型组大鼠血清白介素?6(IL?6)、骨性特异性磷酸酶(BALP)、抗酒石酸酸性磷酸酶(TRACP?5b)、Ⅰ型胶原C端肽(CTX?I)明显降低(P<0.05),骨钙素(OCN)、25羟基维生素D(25?HVD)明显升高(P<0.05)。体外实验表明雷奈酸锶可促进成骨细胞增殖。结论雷奈酸锶可促进成骨细胞增殖,改善骨形态及多项血清指标,对激素性骨质疏松有一定治疗作用。Objective To investigate the effect of strontium ranelate on dexamethasone?induced osteoporosis in rats.Methods To?tally 30 female SD(Sprague?Dawley)rats were randomly divided into 5 groups:normal group,osteoporosis model group,high?dose group,medium?dose group and low?dose group.After 5 weeks,bone morphological and serum indices were detected,and the effects of different concentrations of strontium ranelate on the proliferation of osteoblasts in vitro were investigated.Results Compared with the model group(38.00±2.36)μm,the average trabecular bone thickness[(48.50±4.05)μm,(52.00±4.44)μm]was significant?ly increased(P<0.05).Compared with the model group(189.90±19.27)μm,the trabecular space[(154.26±13.86)μm,(150.02±20.86)μm]was significantly decreased(P<0.05)in the high/medium dose group which indicated that strontium ranelate could significantly improve the above indices to evaluate the bone microstructure.The levels of serum IL?6,BALP,5b(TRACP 5b)and CTX?I in the high dose group were significantly lower than those in the model group(P<0.05),and the osteocalcin and 25?hy?droxyvitamin D were significantly increased(P<0.05).In vitro experiment shows that strontium ranelate can promote osteoblast proliferation.Conclusion Strontium ranelate can promote the proliferation of osteoblasts and improve the bone morphology and a number of serum indices,which have definite treatment action on osteoporosis.

关 键 词：雷奈酸锶 骨质疏松 大鼠 细胞增殖 骨形态学 

分 类 号：R28[医药卫生—中药学]