翻译组研究技术进展  

Advances in the Techniques of Studying Translatome

作　　者：魏康宁 崔俊霞 王梦蕾 王丽[1] 李用芳[1] WEI Kang-ning;CUI Jun-xia;WANG Meng-lei;WANG Li;LI Yong-fang(College of Life Sciences,Henan Normal University,Xinxiang 453007)

机构地区：[1]河南师范大学生命科学学院

出　　处：《生物技术通报》2019年第7期222-229,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31771703,31601241)

摘　　要：基于高通量测序的RNA-Seq广泛被用于检测组织和细胞中基因的表达和调控机理研究,然而已有的文献表明mRNA水平并不能反映蛋白质的丰度,而研究正在翻译的mRNA的翻译组则比转录组更能反映蛋白组的实际水平,因此翻译组研究比转录组研究更具有理论和实践意义。对翻译组研究最为经典的多聚核糖体图谱技术到最近发展起来的核糖体图谱技术、核糖体亲和纯化技术以及翻译谱分析技术进行了综述比较,并介绍了翻译组研究前景,以便为翻译组研究提供参考。High-throughput sequencing-based RNA-Seq has been widely used to detect the expression and regulation mechanism of genes in tissues and cells.However,published literature have shown that mRNA level cannot reflect the real abundance of the related protein,while translatome that studies the being-translated mRNAs is more correlated to the actual abundance of proteins than the transcriptome.Therefore,studying translatome is more theoretical and practical meaning than studying transcriptome.In this paper,we reviewed the main techniques in studying translatome,from the most classical polysome profiling to the recently developed ribosome profiling,ribosomal affinity-purification,and RNC-RNA-Seq,and further compared the advantages and limitations of each technique.We also briefly discussed the prospect of studying translatome for providing a reference in studying translatome.

关 键 词：翻译组 多聚核糖体图谱 核糖体亲和纯化技术 核糖体图谱 翻译谱分析技术 

分 类 号：H31[语言文字—英语]