外源褪黑素对NaCl胁迫下扁穗雀麦种子萌发及幼苗的影响  

Effects of exogenous melatonin on seed germination and seedlings of rescuegrass under NaCl stress

作　　者：熊艳丽 熊毅 赵文达 杨晓鹏[1] 雷雄 余青青 马啸[1] 张新全[1] XIONG Yanli;XIONG Yi;ZHAO Wenda;YANG Xiaopeng;LEI Xiong;YU Qingqing;MA Xiao;ZHANG Xinquan(Department of Grassland Science,Sichuan Agriculture University,Chengdu 611130,Sichuan,China)

机构地区：[1]四川农业大学动物科技学院草业科学系

出　　处：《草业科学》2019年第8期2042-2049,共8页Pratacultural Science

基　　金：四川省科技厅饲草育种攻关(2016 NZ0098 G11);四川省肉牛产业体系创新团队(2017 CXTD02 G7);四川省科技支撑计划(2015SZ0200)

摘　　要：本研究以扁穗雀麦品种‘川西’为试验材料,探讨了外源褪黑素(melatonin,MT)对盐胁迫下扁穗雀麦(Bromus catharticus)种子萌发及幼苗期的形态和生理特性的影响,为提高扁穗雀麦抗盐性提供理论依据。结果表明:外源添加100μmol·L–1褪黑素时扁穗雀麦具有最高的发芽率与抗盐性,此时供试材料的发芽率、发芽势以及幼苗中可溶性蛋白、游离脯氨酸(Pro)含量和过氧化物酶(POD)活性分别较胁迫组提高了22.39%、58.83%、21.78%、121.44%和81.02%,丙二醛(MDA)含量较胁迫组降低了45.43%。研究发现,外源添加褪黑素可提高扁穗雀麦抗氧化酶活性与降低膜质过氧化作用,增加扁穗雀麦幼苗对盐胁迫的耐性。研究结果对扁穗雀麦在盐碱地分布区域的栽培利用具有一定的技术参考价值。The effect of exogenous melatonin on the germination and the morphological and physiological characteristics of the seedling stage of Bromus catharticus‘Chuanxi’(rescuegrass)under salt stress was investigated in the current study,providing a theoretical basis for improving the salt-resistance of‘Chuanxi’.The experimental results indicated that the highest germination rate and salt resistance were obtained when 100μmol·L–1 melatonin was added.In this case,the germination rate,germination energy,soluble protein,free proline(Pro)content,and peroxidase(POD)activity of‘Chuanxi’seedlings increased by 22.39%,58.83%,21.78%,121.44%,and 81.02%,respectively.The malondialdehyde(MDA)content decreased by 45.43%compared to the salt stress treatment.The results suggested that exogenous melatonin(MT)can improve the antioxidant enzyme activity and decrease the membrane peroxidation,thereby improving the adaptability of‘Chuanxi’under salt stress.The research results provided technical improvements for the cultivation and utilization of the‘Chuanxi’in the saline area.

关 键 词：扁穗雀麦 种子萌发 NACL胁迫 褪黑素 

分 类 号：S543.803.4[农业科学—作物学]