Screening of Semi-solid Culture Conditions of Armillaria mellea A9  

作　　者：Qi ZHANG Zhaofeng WANG Qiuying WANG 

机构地区：[1]Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China [2]Xinjiang Agricultural University,Urumqi 830052,China

出　　处：《Agricultural Biotechnology》2019年第4期19-25,共7页农业生物技术（英文版）

摘　　要：[Objectives]This study was conducted to optimize the germplasm resources of Gastrodia elata and improve the condition of declining G.elata production.[Methods]A scientific and reasonable method was used to screen the optimum nutritional conditions(carbon sources,nitrogen sources,microelements,vitamins)and environmental conditions(pH,temperature,light)of Armillaria mellea A9 growth,so as to screen the medium suitable for the growth of A.mellea.[Results]Under the semi-solid culture condition,the suitable pH range of A.mellea mycelia was 4.0-9.0;the temperature was about 25℃;and the light condition was dark culture.Under the experimental conditions,the optimal carbon source was 25 g/L ethanol;the optimal nitrogen source was 2.5 g/L soy peptone;and the optimum microelement and vitamin were 0.5 g/L MnSO 4,0.5 g/L MgSO 4 and 20 mg/L vitamin B6,respectively.After screening,the growth rate of A.mellea rhizomorph was as high as 13.52 mm/d,and the dry weight of mycelium also reached 0.296 7 g.[Conclusions]This study not only omitted a lot of tedious experiments,but also obtained reliable and scientific experimental results,and achieved the purpose of rejuvenating A.mellea,which is conducive to the further development and utilization of G.elata and its symbiotic fungi.[Objectives] This study was conducted to optimize the germplasm resources of Gastrodia elata and improve the condition of declining G. elata production. [Methods] A scientific and reasonable method was used to screen the optimum nutritional conditions（carbon sources, nitrogen sources, microelements, vitamins） and environmental conditions（pH, temperature, light） of Armillaria mellea A9 growth, so as to screen the medium suitable for the growth of A. mellea. [Results] Under the semi-solid culture condition, the suitable pH range of A. mellea mycelia was 4.0-9.0; the temperature was about 25 ℃; and the light condition was dark culture. Under the experimental conditions, the optimal carbon source was 25 g/L ethanol; the optimal nitrogen source was 2.5 g/L soy peptone; and the optimum microelement and vitamin were 0.5 g/L MnSO4, 0.5 g/L MgSO4 and 20 mg/L vitamin B6, respectively. After screening, the growth rate of A. mellea rhizomorph was as high as 13.52 mm/d, and the dry weight of mycelium also reached 0.296 7 g. [Conclusions] This study not only omitted a lot of tedious experiments, but also obtained reliable and scientific experimental results, and achieved the purpose of rejuvenating A. mellea, which is conducive to the further development and utilization of G. elata and its symbiotic fungi.

关 键 词：ARMILLARIA MELLEA mycelial rhizomorph SEMI-SOLID culture Growth rate DRY WEIGHT 

分 类 号：TG1[金属学及工艺—金属学]