JARID2、E-cadherin在卵巢子宫内膜异位症组织中的表达及意义  

Expression and significance of JARID2 and E-cadherin in the ovarian endometriosis tissues

作　　者：钱文艳 江雯波[1] 朱艳[1] 邓伟[2] 周琴[1] QIAN Wen-yan;JIANG Wen-bo;ZHU Yan;DENG Wei;ZHOU Qin(Department of Gynecology and Obstetrics,Kunshan Hospital of Traditional Chinese Medicine,Kunshan 215300,Jiangsu,CHINA;Kunshan Hospital of Traditional Chinese Medicine,Kunshan 215300,Jiangsu,CHINA)

机构地区：[1]昆山市中医医院妇产科,江苏昆山215300 [2]昆山市中医医院病理科,江苏昆山215300

出　　处：《海南医学》2019年第16期2095-2098,共4页Hainan Medical Journal

基　　金：江苏省苏州市科技发展计划(应用基础研究-医疗卫生第二批）项目(编号：SYSD2015027)

摘　　要：目的探索JARID2(Jumonji-ARID2)基因、钙黏附蛋白E(E-cadherin)基因在卵巢子宫内膜异位症在位、异位内膜组织中的表达及意义。方法收集昆山市中医医院妇科2016年1月1日至12月31日因卵巢子宫内膜异位囊肿行经腹腔镜或经腹卵巢囊肿剥除术的71例患者的在位和异位内膜组织,30例因子宫肌瘤行全子宫切除术患者的正常子宫内膜组织。运用免疫组化方法检测JARID2、E-cadherin的表达,分析JARID2的表达与患者临床资料的关系,以及JARID2的表达与E-cadherin表达的相关性。结果卵巢子宫内膜异位症患者的在位内膜、异位内膜组织中JARID2表达阳性率分别为81.69%(58/71)、83.10%(59/71),明显高于正常子宫内膜组织中的26.67%(8/30),差异具有统计学意义(P<0.05);E-cadherin在卵巢子宫内膜异位症患者的在位内膜、异位内膜组织均有表达,其中弱阳性表达率分别为77.46%(55/71),73.24%(52/71),阳性表达率分别为22.54%(16/71)、26.76%(19/71),均显著低于对照组的100%(30/30),差异均具有统计学意义(P<0.05);JARID2的高表达与E-cadherin的低表达呈负相关(r=-0.75,P<0.01)。结论JARID2可能是影响卵巢子宫内膜异位症发生发展的新的关键基因。Objective To evaluate the expression and significance of E-cadherin and Jumonji-ARID2(JARID22)in the ectopic and eutopic endometrium tissues of ovarian endometriosis.Methods From January 1,2016 to December 31,2016,ectopic and eutopic endometrium tissue samples were collected from 71 patients with ovarian endometriosis who underwent laparoscopic or abdominal ovarian cyst exfoliation in Kunshan Hospital of Traditional Chinese Medicine,and normal endometrial tissues were collected from 30 patients with uterine leiomyoma who underwent total hysterectomy.The expression of JARID2 and E-cadherin was detected by immunohistochemistry.Correlation analysis was performed between the expression of JARID2 and clinical data,and between the expression of JARID2 and E-cadherin.Results The positive rates of JARID2 expression in eutopic endometrium and ectopic endometrium tissue samples of patients with ovarian endometriosis were 81.69%(58/71)and 83.10%(59/71),respectively,which were significantly higher than 26.67%(8/30)in normal endometrial tissue.E-cadherin was expressed in eutopic endometrium and ectopic endometrium tissue of patients with ovarian endometriosis.The weak positive rate was 77.46%(55/71),73.24%(52/71),and the positive rate was 22.54%(16/71)and 26.76%(19/71),respectively,which were significantly lower than 100%(30/30)of the normal endometrial tissue(P<0.05).The high expression of JARID2 was negatively correlated with the low expression of E-cadherin(r=-0.75,P<0.01).Conclusion JARID2 may be a new key gene in the pathogenesis of ovarian endometriosis.

关 键 词：卵巢子宫内膜异位症 在位内膜 异位内膜 上皮间质转化 JARID2基因 E-CADHERIN基因 

分 类 号：R711.71[医药卫生—妇产科学]