肺纤方对博来霉素诱导肺纤维化大鼠wnt3a、β-catenin、WIF1和LRP表达的影响  

Effects of Feixian Formula on Expressions of wnt3a, β-catenin, WIF1 and LRP in Bleomycin-induced Pulmonary Interstitial Fibrosis in Rats

作　　者：董环 张晓梅[2] 孟丽红 顾潇枫 于小林[1] 刘彧杉 闫宏 DONG Huan;ZHANG Xiaomei;MENG Lihong;GU Xiaofeng;YU Xiaolin;LIU Yushan;YAN Hong(Beijing University of Chinese Medicine,Beijing 100029,China;Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《中国中医药信息杂志》2019年第9期60-64,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81273696);北京中医药大学自主课题(2019-JYB-XS-205)

摘　　要：目的观察肺纤方对博来霉素诱导的肺纤维化大鼠wnt3a、β-catenin、WIF1、LRP表达的影响,探讨其抗肺纤维化的作用机制。方法采用气管插管滴注博来霉素制作肺纤维化大鼠模型。将120只雄性Wistar大鼠随机分为假手术组、模型组、泼尼松组和肺纤方高、中、低剂量组,造模次日给予相应药物灌胃。于造模后第14、28日分2批进行动物处理,Western blot检测wnt3a、β-catenin、WIF1的蛋白表达,RT-PCR检测LRP的mRNA表达。结果与假手术组比较,第14、28日模型组大鼠肺组织wnt3a、β-catenin、LRP表达均显著升高(P<0.05,P<0.01),第28日WIF1表达显著降低(P<0.05);与模型组比较,肺纤方中剂量组第14、28日大鼠肺组织wnt3a、β-catenin、LRP表达显著降低(P<0.05,P<0.01),WIF1表达显著升高(P<0.05,P<0.01)。结论肺纤方具有抗肺纤维化的作用,其机制可能与抑制模型大鼠wnt3a、β-catenin、LRP的过表达和增加WIF1的低表达有关。Objective To investigate the effects of Feixian Formula on the expressions of wnt3a,β-catenin,WIF1 and LRP in bleomycin(BLM)-induced pulmonary fibrosis in rats;To explore its anti-pulmonary fibrosis mechanism.Methods Intravenous intubation of BLM was used to establish pulmonary fibrosis rat models.120 Wistar rats were randomly divided into sham-operation group,model group,Prednisone group,and Feixian Formula high-,mediumand low-dosage groups.The day after model establishing,gavage was intervened with corresponding medicine.Animals were treated in two batches on the 14th and 28th day after modeling.The protein expressions of wnt3a,β-catenin and WIF1 were detected by Western blot,and the mRNA expression of LRP was detected by RT-PCR.Results Compared with sham-operation group,the expressions of wnt3a,β-catenin and LRP in lung tissue of rats in model group increased significantly on day 14 and day 28(P<0.05,P<0.01),and the expression of WIF1 decreased significantly on day 28(P<0.05).Compared with model group,the expressions of wnt3a,β-catenin and LRP in lung tissue of rats in Feixian Formula medium-dosage group decreased significantly on day 14 and day 28(P<0.05.P<0.01),and the expression of WIF1 increased significantly(P<0.05,P<0.01).Conclusion Feixian Formula has anti-pulmonary fibrosis effects,and the mechanism may be related to inhibiting the overexpression of wnt3a,β-catenin and LRP in rats with pulmonary fibrosis and increasing the low expression of WIF1.

关 键 词：肺纤方 博来霉素 肺纤维化 WNT/Β-CATENIN信号通路 大鼠 

分 类 号：R285.5[医药卫生—中药学]