枳实黄酮对照提取物研究及其在枳实药材质量控制中的应用  

Study on Flavonoids Reference Extracts and Its Application to Quality Control of Aurantii Fructus Immaturus

作　　者：杨丽丽 姜艳艳[1] 蒋丽娟 张薇 张永文 刘斌[1] YANG Lili;JIANG Yanyan;JIANG Lijuan;ZHANG Wei;ZHANG Yongwen;LIU Bin(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Center for Drug Evaluation,National Medical Products Administration,Beijing 100022,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]国家药品监督管理局药品审评中心,北京100022

出　　处：《中国中医药信息杂志》2019年第9期73-79,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京中医药大学基本科研业务费项目(2018-JYBZZ-XJSJJ007)

摘　　要：目的建立枳实黄酮对照提取物制备方法及定量方法,并探索其在枳实药材质量控制中应用的可行性。方法采用溶剂提取法、色谱分离方法制备枳实黄酮对照提取物,以芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷对照品为对照,对枳实黄酮对照提取物含量进行标定。分别以已知含量的枳实黄酮对照提取物和单体成分对照品(芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷)为对照,建立枳实药材HPLC含量测定方法,并测定枳实药材中4种成分的含量,采用t检验和相关性分析对含量测定结果进行统计分析。结果以枳实黄酮对照提取物为对照,芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷进样量在各自范围内与峰面积呈良好的线性关系(r>0.999 6),平均回收率为100.45%~101.27%。t检验结果显示,2种方法测定的芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷含量P值分别为0.97、0.38、0.69、0.84;相关性分析显示,2种方法测定结果的相关系数均在0.95以上,表明2种方法无明显差异。结论枳实黄酮对照提取物可用于枳实药材的质量控制,本研究为枳实药材的质量控制与评价提供了新方法。Objective To establish preparation method and quantitative method of flavonoids reference extracts(FRE);To explore the feasibility of its application to quality control of Aurantii Fructus Immaturus.Methods The FRE of Aurantii Fructus Immaturus was prepared by solvent extraction and chromatographic separation techniques.Narirutin,naringin,hesperidin and neohesperidin were set as references to calibrate the contents of FRE of Aurantii Fructus Immaturus.Setting the known contents of FRE of Aurantii Fructus Immaturus and single components(Narirutin,naringin,hesperidin and neohesperidin)as references,HPLC methods for Aurantii Fructus Immaturus were established to detect the four components in Aurantii Fructus Immaturus.The results of the content determination were statistically analyzed by t-test and correlation analysis.Results Setting the reference extracts as references,Narirutin,naringin,hesperidin and neohesperidin were in a good linear relationship(r>0.999 6)within each range,and the average recovery rate was 100.45%to 101.27%.The t-test results showed that the P values of two determination methods of narirutin,naringin,hesperidin and neohesperidin were 0.97,0.38,0.69 and 0.84,respectively,and the correlation analysis showed that the correlation coefficients were all above 0.95,indicating that there was no significant difference between the two methods.Conclusion The FRE can be used in the quality control of Aurantii Fructus Immaturus.This study provides a new method for quality control and evaluation of Aurantii Fructus Immaturus.

关 键 词：枳实 对照提取物 黄酮 质量控制 

分 类 号：R284.1[医药卫生—中药学]