交泰丸及其组分含药血清对豚鼠心室肌细胞钠通道的影响  

Effects of Jiaotai Pills and Its Different Components Drug-Containing on Sodium Channel of Guinea-pig′s Ventricular Myocardial Cells

作　　者：邢作英[1] 王永霞[1] 朱明军[1] 宋欢欢[1] 高原[1] 陈鹏[1] 郑佳[1] 陈彦[1] 李彬[1] 安宜沛 XING Zuo-ying;WANG Yong-xia;ZHU Ming-jun;SONG Huan-huan;GAO Yuan;CHEN Peng;ZHENG Jia;CHEN Yan;LI Bin;AN Yi-pei(Department of Heart Center,First Affiliated Hospital,Henan University of Chinese Medicine,Zhengzhou,450000)

机构地区：[1]河南中医药大学第一附属医院心脏中心

出　　处：《中国中西医结合杂志》2019年第8期983-987,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81102698,No.81803944);河南省科技创新杰出青年计划(No.124100510008);郑州市普通科技攻关(No.112PPTSF319-17);河南省科技创新团队支持计划(No.C20130050);河南省高校科技创新团队支持计划(No.131RTSTHN012);河南省2018年科技发展计划(No.182102311152)

摘　　要：目的探讨交泰丸及不同组分含药血清对单个豚鼠心室肌细胞Na^+通道的影响。方法制备含药血清,急性分离豚鼠心室肌细胞,细胞复钙后随机分为空白组、黄连组(黄连100g煎液)、肉桂组(肉桂10 g煎液)、黄连加肉桂组(黄连100g单煎液+等体积肉桂10g单煎液)和交泰丸组(黄连100g+肉桂10g合煎),各组分别加入各含药血清,实验分为10%和15%两个浓度组,加药完成后细胞放于37℃,5%CO2的孵育箱中孵育24 h后进行膜片钳实验,记录Na^+通道电流及其激活和失活曲线。结果与空白组比较,15%浓度黄连组、肉桂组、黄连加肉桂组、交泰丸组均不同程度抑制Na^+电流(P<0.05),其中黄连加肉桂组差异最显著;10%浓度药物组差异无统计学意义(P>0.05)。与空白组比较,15%浓度交泰丸组Na+通道激活和失活时间常数延长(P<0.05)。结论交泰丸及各组分含药血清对钠离子电流有不同程度的抑制作用,与Ⅰ类抗心律失常药物的作用机制相同,这可能是交泰丸抗心律失常的电生理机制。Objective To explore the effects of Jiaotai Pills and its different serum concentrations(10%and 15%)on the sodium channel currents(INa)in guinea-pig ventricular myocytes.Methods Preparing the drug serum and isolating guinea-pig ventricular tissues,the myocytes were divided into five groups randomly:the control group,the Huanglian group(decoction of 100 g Coptis chinensis Franch.),the Rougui group(decoction of 10g Cinnamomum cassia Presl),the Huanglian and Rougui group(single decoction of 100g Coptis chinensis Franch.+equal volumn decoction of 10g Cinnamomum cassia Presl),the Jiaotai Pills group(co-decoction of 100g Coptis chinensis Franch.and 10g Cinnamomum cassia Presl).Cells were divided into 10%and 15%drug concentration groups and incubated at 37℃in a humidified,5%CO2 incubator after 24 h,patch clamp test was performed to record Na+channel current and its activation and inactivation curves ccurves.Results Compared with the control group,15%concentration groups inhibited INa current density to different degrees(all P<0.05),and the highest inhibition was 15%concentration Huanglian and Rougui group;the 10%concentration groups had no significant differences(all P>0.05).Compared with the control group,the 15%concentration Jiaotai Pills prolonged the time courses of activition and inactivition(all P<0.05).Conclusion There were varying degrees of inhibition of Jiaotai Pills decoction and its disassembled prescription in different serum concentration on INa,the effect might be a mechanism of antiarrhythmic action,and it was the same as ClassⅠantiarrhythmic agent.

关 键 词：交泰丸 血清药理学 膜片钳 钠离子通道 动力学参数 

分 类 号：R28[医药卫生—中药学]