虾夷扇贝家系闭壳肌脂肪酸与C/N相关性研究  

THE CORRELATION BETWEEN THE FATTY ACID CONTENT AND THE C/N RATIO IN THE SELECTIVE BREEDING FAMILIES OF JAPANESE SCALLOP MIZUHOPECTEN YESSOENSIS

作　　者：谢玺[1] 王庆志[1] 滕炜鸣[1] 刘项峰[1] 于佐安[1] 李华琳[1] 周遵春[1] XIE Xi;WANG Qing-Zhi;TENG Wei-Ming;LIU Xiang-Feng;YU Zuo-An;LI Hua-Lin;ZHOU Zun-Chun(Liaoning Ocean and Fisheries Science Research Institute,Dalian Key Laboratory of Genetic Resources for Marine Shellfish,Dalian 116023,China)

机构地区：[1]辽宁省海洋水产科学研究院大连市海产贝类种质资源创新利用重点实验室

出　　处：《水生生物学报》2019年第5期1062-1071,共10页Acta Hydrobiologica Sinica

基　　金：辽宁省重点研发计划(2017203006);国家自然科学基金(31302186);大连市高层次创新人才支持计划(2016RQ081);辽宁省渔业科技计划(201818和201826);现代农业体系建设专项(CARS-49);辽宁省博士科研启动基金(201601387)资助~~

摘　　要：以虾夷扇贝(Mizuhopecten yessoensis)的生长性状、闭壳肌脂肪酸组成与含量、闭壳肌C、N元素含量及其比值为主要考察指标,在对比选育家系的生长性状差异的基础上,分析了不同生长速度的虾夷扇贝家系闭壳肌脂肪酸组成与含量差异,研究了闭壳肌C、N元素含量及其C/N比值与脂肪酸含量的相关性。结果表明,不同扇贝家系闭壳肌的主要脂肪酸含量差异较小而相对丰度差异明显,EPA(3.59—4.09 mg/g)和DHA(3.03—3.46 mg/g)之和占比扇贝闭壳肌脂肪酸总量的47.19%—49.32%;不同家系的虾夷扇贝闭壳肌C、N元素稳态性较好,C元素和N元素的含量分别为(39.21±0.04)%和(10.62±0.16)%,C/N比值为3.70±0.06;相关分析表明家系闭壳肌C/N比值与TFA、SFA、PUFA、EPA以及DHA等脂肪酸含量呈显著负相关。因此,在进行虾夷扇贝生长相关选择育种时,可以结合闭壳肌脂肪酸组成与含量、闭壳肌元素C/N比值开展复合性状选育,从而为扇贝闭壳肌高品质脂肪酸家系选育提供科学依据。Family selection has emerged as a crucial tool for selective breeding in aquaculture.The current artificial selection for Japanese scallop(Mizuhopecten yessoensis)predominantly focused on the apparent characters,e.g.growth,shell color and survival rate.Little studies of selective breeding were based on nutritional value of adductor muscle.The present study investigated the growth performance,the fatty acid composition,the content of carbon and nitrogen and C/N ratio of adductor muscle in distinct selective breeding families of M.yessoensis and explored the correlation between C/N ratio and fatty acid content.The results demonstrated that the growth features of the families 25#,54#and 19#were superior to those of 32#,87#and 93#(P<0.05).No significant difference of the total major fatty acids was observed between distinct families,but the relative abundance of the major fatty acids was obviously different between distinct families as PUFA>SFA>MUFA.The amounts of EPA and DHA based on dry weight of adductor muscle in M.yessoensis varied from 3.59 to 4.09 mg/g,and from 3.03 to 3.46 mg/g,respectively.The relative abundance of both EPA and DHA ranged between 47.19%and 49.32%of the total fatty acids.Moreover,the content of carbon and nitrogen of adductor muscle in distinct families of M.yessoensis remained constantly with(39.21±0.04)%and(10.62±0.16)%,respectively.The C/N ratio was(3.70±0.06).The further correlation analysis showed that the C/N ratio was positively related to the growth of adductor muscle in distinct families.On the contrary,a prominently negative correlation existed between the C/N ratio and the content of TFA,SFA,PUFA,EPA or DHA with the most evident one between C/N ration with DHA.Our results indicated that the fatty acid composition,the content of C and N and the C/N ratio of adductor muscle could be used for selective breeding of M.yessoensis.These findings provide important implications for the broad development of the selective breeding in scallops.

关 键 词：虾夷扇贝 选择育种 闭壳肌 脂肪酸 C/N比值 

分 类 号：S968.3[农业科学—水产养殖]