吴李碘泡虫的重描述及其长江流域不同江段株系的比较研究  

REDESCRIPTION OF MYXOBOLUS WULII AND COMPARISON OF ITS STRAINS IN DIFFERENT SECTIONS OF THE YANGTZE RIVER BASIN

作　　者：杨承忠 张雕雕 赵元莙 YANG Cheng-Zhong;ZHANG Diao-Diao;ZHAO Yuan-Jun(Chongqing Key Laboratory of Animal Biology,College of Life Sciences,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学生命科学学院重庆市动物生物学重点实验室

出　　处：《水生生物学报》2019年第5期1092-1097,共6页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31471980和31501845);重庆市科委基金项目(cstc2017jcyjAX0165和cstc2018jcyjAX0738);重庆市留创计划创新项目(cx2018108)资助~~

摘　　要：研究在对吴李碘泡虫Myxobolus wulii(Wu&Li)Landsberg&Lom,1991重描述的基础上,基于形态和分子数据对长江流域不同江段的吴李碘泡虫(重庆株系、湖北株系及江苏株系)进行了比较研究。结果表明:吴李碘泡虫重庆株系孢子及极囊量度比湖北株系略小,重庆株系两极囊等大而湖北株系两极囊大小不等。重庆株系、湖北株系及江苏株系18S rDNA序列相似度为99.2%—99.9%,遗传距离为0.002—0.007。系统发育分析显示:吴李碘泡虫并未形成地理种群特有的进化枝,也并未依宿主种类而聚支,而是依据寄生部位不同分为鳃寄生和肝胰脏寄生2大支系。这表明,相同寄生部位的吴李碘泡虫具有更近的亲缘关系。吴李碘泡虫的2大支系中,鳃寄生种群先分化出来,这可能与体表寄生和体内寄生的演化有关,而鳃寄生的吴李碘泡虫可能是较早定居的群体。This study re-described Myxobolus wulii(Wu&Li)Landsberg&Lom,1991 and compared its strains in different sections of Yangtze River Basin(Chongqing strain,Hubei strain and Jiangsu strain)based on morphological and molecular(18S rDNA)data.The results showed that the spores and polar capsules of Chongqing strain were slightly smaller than those of Hubei strain,and that polar capsules and spores of Chongqing strain were equal in size while they were unequal for Hubei strain.The similarities and genetic distances among the three strains of M.wulii were 99.2%—99.9%and 0.002—0.007,respectively.Phylogenetic analysis showed that the sequences of M.wulii formed a poor geographical structure as well as poor host-original structure,but a strong parasitic site structure.The sequences of M.wulii clustered into two lineages:gill-parasites clade and hepatopancreas-parasites clade,indicating that the individuals of M.wulii have closer relationship with the same site of host.Moreover,the gill-parasites clade diverged earlier than hepatopancreas-parasites clade,which might be related to the evolution of external-parasites to internal-parasites.These data inferred that the gill-parasites population might be the earliest migrates of M.wulii.

关 键 词：吴李碘泡虫 重描述 长江流域 株系比较 18S RDNA 

分 类 号：S941.51[农业科学—水产养殖]