分子印迹光子晶体凝胶膜可视化检测葡萄酒中邻氨基苯甲酸甲酯  

Molecularly Imprinted Photonic Hydrogels for Visual Detection of Methylanthranilate in Wine

作　　者：吴伟珍 黄梦霞 黄庆达 吕彩华 赖家平[1] 孙慧[2] WU Wei-Zhen;HUANG Meng-Xia;HUANG Qing-Da;LYU Cai-Hua;LAI Jia-Ping;SUN Hui(School of Chemistry&Environment,South China Normal University,Guangzhou 510006,China;College of Environmental Science&Engineering,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]华南师范大学化学与环境学院,广州510006 [2]广州大学环境科学与工程学院,广州510006

出　　处：《分析化学》2019年第9期1330-1336,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然基金项目(Nos.21677053,21876033)资助~~

摘　　要：邻氨基苯甲酸甲酯(Methylanthranilate,MA)是我国禁止在葡萄酒中添加的香料,对MA测定是葡萄酒中重要的质控环节。因此,开发快速检测葡萄酒中违禁香料的方法具有重要的实际意义。本研究利用分子印迹技术与光子晶体技术相结合,以MA为模板分子、甲基丙烯酸(MAA)为功能单体、甲基丙烯酸乙二醇酯(EGDMA)为交联剂,在60℃加热条件下引发聚合,去除光子晶体模板和洗脱印迹分子后得到反蛋白石结构分子印迹光子晶体凝胶膜。研究结果表明,此印迹凝胶膜对MA分子有较好的选择性,在分子识别过程中MA印迹凝胶膜的Bragg衍射峰位置随着MA浓度的增大而发生红移,达到平衡状态的时间为6 min,且偏移值(Δλ)与MA的浓度(C)在0.1~10.0 mmol/L范围内有良好的线性关系,检出限为31μmol/L,可实现重复循环检测使用。此外,在检测不同浓度印迹分子时,肉眼能够观察到印迹凝胶膜颜色的变化。此智能型凝胶膜可望用于葡萄酒中MA的可视化快速检测。Methylanthranilate(MA)is a kind of spice illegally added in the wine in China.The determination of MA in wine is an important quality control link.Therefore,it is of great significance to develop a rapid method for detection of prohibited spice in wine.In this work,an inverse opal structural molecularly imprinted photonic hydrogels(MIPHs)membrane was fabricated by using the photonic crystal techniques and molecular imprinting techniques.The MIPHs membrane was prepared by using methylanthranilate as template molecule,methacrylic acid as functional monomer,ethylene glycol dimethacrylate as cross linker,followed by a thermal polymerization at 60℃.Then the inverse opal structural MIPHs membrane was obtained after removing photonic crystal template and imprinted molecules.The results showed that the MIPHs membrane exhibited a good selectivity property to MA,and it responded to MA within 6 min.The Bragg diffraction peak shifted with increasing concentration of MA.A linear relationship was found between Δλ and the concentration of MA in the range from 0.1 mmol/L to 10 mmol/L.And the least detectable concentration was about 31μmol/L.Furthermore,a color change of the MIPHs membrane couldbe observed by naked eyes.Therefore,the smart MIPHs membrane showed great potential in rapid and visual detection of MA in wine.

关 键 词：分子印迹 光子晶体 可视化检测 邻氨基苯甲酸甲酯 

分 类 号：R28[医药卫生—中药学]