针灸传感针的研制及在中药活性成分调控细胞一氧化氮释放检测中的应用研究  

Study on preparation of sensing acupuncture needle and its applications in monitoring nitric oxide release in the regulation of active constituents of traditional Chinese medicine

作　　者：唐丽娜 李玉桃 梁凤霞 王华 张国军 TANG Li-na;LI Yu-tao;LIANG Feng-xia;WANG Hua;ZHANG Guo-jun(Center for Nano-Biosensing of School of Laboratory Medicine,Hubei University of Chinese Medicine,Wuhan 430065,China;Hubei Provincial Collaborative Innovation Center of Preventive Treatment,Wuhan 430065,China;Department of Acupuncture and Moxibustion,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学检验学院纳米生物传感中心,武汉430065 [2]湖北省针灸治未病协同创新中心,武汉430065 [3]湖北中医药大学针灸骨伤学院,武汉430065

出　　处：《中华中医药杂志》2019年第8期3435-3439,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖北省教育厅指导性项目(No.B2017100)~~

摘　　要：目的:探讨中药活性成分对一氧化氮(NO)作用以及高灵敏度检测NO浓度的方法。方法:将纳米生物传感技术与传统针灸针结合制备针灸传感针,将其用于检测药物作用下细胞生物活性分子NO水平,同时采用试剂盒方法检测不同药物培养下细胞NO释放水平。结果:针灸传感针制备成功;针灸传感针用于细胞水平NO检测,其结果与试剂盒方法一致,青藤碱对内皮细胞中NO释放有抑制的作用,而大蒜素对细胞中NO释放有促进的作用。结论:针灸传感针成功监测细胞内NO,既反映中药活性成分的调节作用,又为探讨中药作用机制提供理论和技术支持。Objective: To explore the influence of nitric oxide(NO) with active constituents of traditional Chinese medicine(TCM) and the highly sensitivity determination method of NO concentration. Methods: A unique nano-biosensing platform is expected by combining modern nanotechnology with traditional acupuncture needle to prepare sensing needle, which can be applied in detecting bioactive molecules NO in cell. Results: The sensing acupuncture needle was prepared successfully and then used to monitor NO release regulated by TCM by electrochemical methods. And it was seen that sinomenine inhibited NO release in cells, and allicin promoted NO release in cells, which was consistent with that of the NO commercial kit. Conclusion:This sensing needle is successfully applied in realizing detection of NO in cell, which not only can reflect the neurotransmitter effect regulated by TCM, but also can provide theoretical and technical support for the mechanism of TCM therapy.

关 键 词：生物传感器 纳米技术 针灸针 一氧化氮 中药调节 

分 类 号：R24[医药卫生—中医临床基础]