疏肝健脾活血方对Notch-Hes 1信号通路的影响  

Effects of Shugan Jianpi Huoxue Decoction on Notch-Hes 1 signal pathway

作　　者：黄棪 鲁军 夏雪皎[1] 杨东升 张俊杰[1] HUANG Yan;LU Jun;XIA Xue-jiao;YANG Dong-sheng;ZHANG Jun-jie(Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学

出　　处：《中华中医药杂志》2019年第8期3443-3446,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81473647)~~

摘　　要：目的:观察疏肝健脾活血方在肝星状细胞(HSC)/肝窦内皮细胞(SEC)共培养体系下对Notch信号通路的影响,探讨疏肝健脾活血方抗肝纤维化的作用机制。方法:制备疏肝健脾活血方含药血清;建立大鼠肝纤维化模型;分离正常大鼠HSC、SEC及肝纤维化大鼠肝窦内皮细胞,将HSC与SEC共培养,先以rhNF-κB激活或DAPT阻断Notch信号通路再用含药血清孵育,Western Blot法及RT-PCR法检测HSC中Notch 1、Jagged 1、Hes 1蛋白及mRNA表达量。结果:与模型组比较,中药组Notch 1蛋白及Notch 1、Jagged 1、Hes 1 mRNA表达量下降(P<0.01);与NF组比较,中药加NF组Notch 1、Jagged 1、Hes 1蛋白及mRNA表达量均下降(P<0.05,P<0.01);与DAPT组比较,中药加DAPT组Notch 1、Jagged 1蛋白表达量下降(P<0.01)。结论:疏肝健脾活血方可能通过降低Notch 1、Jagged 1、Hes 1蛋白及mRNA表达量抑制HSC活化从而起到抗肝纤维化的作用。Objective: To observe the effects of Shugan Jianpi Huoxue Decoction(SJHD) on Notch signaling pathway in co-culture of sinusoidal endothelial cell(SEC) and hepatic stellate cell(HSC) for exploring the mechanism of SJHD in anti-hepatic fibrosis. Methods: Drug-containing serum of SJHR was prepared. Hepatic fibrosis model in rats was established. The normal rat HSC, SEC and sinusoidal endothelial cells of hepatic fibrosis rats were isolated. HSC and SEC were co-cultured. Notch signaling pathway was activated by rhNF-kappa B or blocked by DAPT, and then incubated with medicated serum. Western Blot and RTPCR methods were used to detect the expression of Notch 1, Jagged 1, Hes 1 proteins and mRNA. Results: Compared with the model group, the expression of Notch 1 protein and Notch 1, Jagged 1, Hes 1 mRNA in SJHP group was decreased(P<0.01).Compared with the NF group, the expression of Notch 1, Jagged 1, Hes 1 protein and mRNA in SJHD and NF group was decreased(P<0.05, P<0.01). Compared with the DAPT group, the expression of Notch 1, Jagged 1 protein in SJHD and DAPT group was decreased(P<0.01). Conclusion: It is the possible anti-hepatic fibrosis mechanism that SJHD can reduce the proteins and mRNA expression of Notch 1, Jagged 1, Hes 1 in HSC for inhibiting HSC activation.

关 键 词：肝纤维化 肝星状细胞 肝窦内皮细胞 NOTCH信号通路 

分 类 号：R28[医药卫生—中药学]