IL-28B基因多态性在丙型肝炎患者中的分布及临床意义  

Distribution and clinical significance of IL-28B gene polymorphism in patients with chronic hepatitis C

作　　者：罗庆元[1] 吕健宏 李高波 蒋莹[2] LUO Qingyuan;L Jianhong;LI Gaobo;JIANG Ying(Department of Gastroenterology,Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063,China;Department of Infection,Second Affiliated Hospital of Xinjiang Medical University)

机构地区：[1]新疆医科大学第二附属医院消化二科,乌鲁木齐830063 [2]新疆医科大学第二附属医院感染科

出　　处：《山西医科大学学报》2019年第8期1066-1069,共4页Journal of Shanxi Medical University

基　　金：新疆维吾尔自治区自然科学基金资助项目(2016D01C197)

摘　　要：目的探讨白细胞介素28B(interleukin-28B,IL-28B)基因多态性表达及意义。方法采用实时荧光定量PCR(qRT-PCR)检测126例丙肝患者外周血清中HCV-RNA的表达,用基因测序的方法分析获得患者IL-28B的rs12979860和rs12980275基因位点多态性;比较IL-28B基因多态性在汉族和维吾尔族表达,及在丙型肝炎和肝硬化之间的差异。结果126例丙肝患者IL-28B基因rs12979860位点基因型和基因频率在肝硬化组与慢性肝炎组中的分布频率差异有统计学意义(P<0.05)。相对于慢性肝炎患者,rs12979860 TT型增加肝硬化的发病风险。维吾尔族患者rs12979860 CT型显著多于汉族。经多变量Logistic回归分析,rs12979860含等位基因T的基因型发生肝硬化的比值比(OR及95%CI)为1.179(0.644-1.357)。IL-28B基因rs12980275位点基因型和基因频率在肝硬化组与慢性肝炎组中的分布频率差异有统计学意义(P<0.05)。相对于慢性肝炎患者,肝硬化rs12980275 AG型显著增加(P<0.05)。rs12979860的基因型在汉族和维吾尔族间对比无统计学意义。Logistic回归分析显示该位点增加肝硬化的患病风险。结论IL-28B基因rs12980275,rs12979860位点基因多态性与肝硬化的发生有相关性,rs12980275在汉族和维吾尔族患者之间存在差异,有助于预测慢性丙肝的临床转归和指导治疗。Objective To investigate the expression and significance of interleukin-28B(IL-28B)gene polymorphism.Methods The expression of HCV-RNA in peripheral serum of 126 patients with hepatitis C was detected by real-time fluorescence quantitative PCR(qRT-PCR).The polymorphisms of rs12979860 and rs12980275 loci of IL-28B were analyzed by gene sequencing.The polymorphisms of IL-28B gene were compared between Han and Uygur nationalities,and the difference between hepatitis C and cirrhosis was statistically compared and analyzed.Results The genotype and genotype frequencies of IL-28B rs12979860 locus in 126 patients with hepatitis C were significantly different between cirrhosis group and chronic hepatitis group(P<0.05).Compared with patients with chronic hepatitis,rs12979860 TT increased the risk of cirrhosis.The type of rs12979860 CT in Uygur patients was significantly higher than that in Han patients.By multivariate logistic regression analysis,the ratio of OR and 95%CI of rs12979860 genotype containing allele T to cirrhosis was 1.179(0.644-1.357).The genotype and genotype frequencies of the rs12980275 locus of IL-28B gene were significantly different between cirrhosis group and chronic hepatitis group(P<0.05).Compared with the patients with chronic hepatitis,the rs12980275 AG type in cirrhosis patients increased significantly(P<0.05).The genotype of rs12979860 had no significant difference between Han and Uygur nationalities.Logistic regression analysis showed that this site increased the risk of cirrhosis.Conclusion The polymorphisms of IL-28B gene rs12980275 and rs12979860 loci are correlated with the occurrence of liver cirrhosis,and rs12980275 is different in Uygur patients of Han nationality,and can be used to predict the clinical outcome of chronic hepatitis C and guide treatment.

关 键 词：慢性丙型肝炎 肝硬化 白介素-28B 基因多态性 

分 类 号：R512.63[医药卫生—内科学]