异丙酚对急性脊髓损伤大鼠神经元凋亡的影响  

Influence of propofol on apoptosis of spinal cord neurons in rats with acute spinal cord injury

作　　者：郝建红 何非[1] 吴赞情 罗振国[1] 董补怀[1] HAO Jianhong;HE Fei;WU Zanqing;LUO Zhenguo;DONG Buhuai(Department of Anesthesiology,Honghui Hospital,Xi’an Jiaotong University,Xi’an 710000,China)

机构地区：[1]西安交通大学医学院附属红会医院麻醉科

出　　处：《山西医科大学学报》2019年第8期1128-1133,共6页Journal of Shanxi Medical University

摘　　要：目的研究异丙酚对急性脊髓损伤大鼠神经元凋亡的影响。方法72只Wistar大鼠,体质量200-250 g,雄性,清洁级,随机分为三组,假手术组、SCI组、异丙酚组,每组24只。假手术组大鼠仅接受手术而不遭受脊髓打击;SCI组和异丙酚组采用改良的Allen’s致伤法建立大鼠急性脊髓损伤模型;异丙酚组的大鼠在脊髓损伤后4 h,经股静脉持续泵注异丙酚1.0 mg/(kg·min),持续1 h。各组分别于术后1,3,5,7 d随机处死6只大鼠,HE染色观察脊髓神经元病理形态学改变,TUNEL法测定脊髓损伤后神经元凋亡,免疫组织化学检测脊髓Caspase-3、AIF的表达。结果与假手术组相比,SCI组、异丙酚组各时点脊髓组织凋亡细胞增多,AIF、Caspase-3表达增加(P<0.01);与SCI组比较,异丙酚组各时点凋亡细胞、AIF和Caspase-3表达明显减少(P<0.05)。结论异丙酚可减轻急性脊髓损伤大鼠神经元凋亡,其机制与抑制脊髓组织AIF、Caspase-3表达有关。Objective To study the influence of propofol on apoptosis of spinal cord neurons in rats with acute spinal cord injury.Methods Totally 72 male Wistar rats,200-250 g,were randomly divided into 3 groups:control group,SCI group,and propofol group.Spinal cord injury models were established by Allen’s method in SCI group and propofol group.In propofol group,propofol 1.0 mg/(kg·min)was injected continuously through femoral vein at 4 h after spinal cord injury for 1 h.Six rats in each group were executed 1 d,3 d,5 d,7 d after operation.HE method was used to detect the pathologic changes of spinal cord,and TUNEL was used to detect the level of apoptosis of neural cells.The expression of AIF,Caspase-3 was detected by immunohistochemical staining.Results Compared with control group,the number of neuronal apoptosis and the protein expression levels of AIF and Caspase-3 were significantly increased in SCI group and propofol group(P<0.01).Compared with SCI group,the markers mentioned above were markedly reduced in propofol group(P<0.05).Conclusion Propofol may attenuate acute spinal cord injury by decreasing AIF and caspase-3 protein expression and inhibiting apoptosis.

关 键 词：异丙酚 脊髓损伤 细胞凋亡 CASPASE-3 凋亡诱导因子 

分 类 号：R614[医药卫生—麻醉学]