河南部分地区湖羊血液无浆体感染情况调查  

Prevalence investigation of Anaplasma in blood of Hu sheep in parts of Henan province

作　　者：宁晓冬 崔艳艳[2] 闫亚群[2] 王晓星 史柯[2] 王坤轮[2] 周永春 宁长申 菅复春[2] NING Xiaodong;CUI Yanyan;YAN Yaqun;WANG Xiaoxing;SHI Ke;WANG Kunlun;ZHOU Yongchun;NING Changshen;JIAN Fuchun(Henan Vocational College of Applied Technology,Zhengzhou 450042,China;College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南应用技术职业学院,河南郑州450042 [2]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业大学学报》2019年第4期563-567,共5页Journal of Henan Agricultural University

基　　金：国家重点研发计划项目(2018YFD0502100);国家现代肉羊产业技术体系项目(nycytx-38)

摘　　要：为了解湖羊血液无浆体病的流行情况,采用巢式PCR对采自河南省驻马店、洛阳、安阳、汝州市共4个地区湖羊的165份血液样品,基于无浆体16S rRNA和MSP4基因进行检测。结果表明,共测出嗜吞噬细胞无浆体(Anaplasma phagocytophilum)、牛无浆体(A.bovis)和绵羊无浆体(A.ovis)3种无浆体,阳性率分别为1.21%(2/165)、9.70%(16/165)和24.85%(41/165);无浆体总阳性率为32.73%(54/165)。表明湖羊无浆体感染普遍存在,不同饲养方式、不同年龄湖羊的无浆体阳性率存在差异。In order to understand the infection situation of Anaplasma in blood of Hu sheep,a total of 165 blood specimens were collected from four cities including Zhumadian,Luoyang,Anyang and Ruzhou in Henan province.Anaplasma was examined based on 16S rRNA and MSP4 gene by the nested PCR.The result showed that three species of Anaplasma were found in blood samples,including A.phagocytophilum,A.bovis,A.ovis,whose infection rate was 1.21%(2/165),9.70%(16/165)and 24.85%(41/158)respectively.The overall infection rate of Anaplasma in blood of Hu Sheep was 32.73%(54/165).The results indicated that Anaplasma infection was ubiquitous in blood of Hu sheep.There were differences in Anaplasma infection of Hu sheep of different feeding patterns and different ages.

关 键 词：湖羊 无浆体 PCR 流行病学 

分 类 号：S855.99[农业科学—临床兽医学]