HIV/HCV混合感染者与HCV感染者感染HCV基因型分析  

Comparison of HCV genotypes in patients with HIV/HCV co-infection and HCV mono-infection in Guangzhou

作　　者：邓浩辉 李晓强 高洪波 陈伟烈 Deng Haohui;Li Xiaoqiang;Gao Hongbo(Department of Liver Diseases,Eighth People's Hospital,Affiliated to Guangzhou Medical University,Guangzhou 510060,Guangdong Province,China)

机构地区：[1]广州医科大学附属市八人民医院重症肝病科,广州市510060 [2]广州医科大学附属市八人民医院传染病研究所,广州市510060

出　　处：《实用肝脏病杂志》2019年第5期656-659,共4页Journal of Practical Hepatology

基　　金：广州市卫生健康科技项目(编号：20191A011037);广州市科信局科技惠民专项(编号：2014Y2-00149)

摘　　要：目的使用二代测序技术检测广州地区人类免疫缺陷病毒(HIV)/丙型肝炎病毒(HCV)混合感染者和HCV感染者HCV基因型,了解本地区HCV基因型的流行特点。方法在233例HCV感染者中,包括95例HIV/HCV混合感染者和138例HCV感染者,使用Illumina Miseq平台PE300模式对HCV Core和NS5B片段进行测序,采用生物信息学分析确定HCV基因型,对测序结果提示混合基因型感染的样本,使用NS5A基因型特异引物进行验证。结果HIV/HCV混合感染者静脉吸毒感染构成比显著高于HCV感染者(77.7%对19.6%P<0.001),HCV感染者输血感染的构成比显著高于HIV/HCV混合感染者(48.6%对3.2%P<0.001);HIV/HCV混合感染者HCV Core基因片段平均数据量为(15456±6689)reads,HCV感染者为(14323±5321)reads,两组无显著差异(P>0.05);HIV/HCV混合感染者HCV NS5B基因片段平均数据量为(16432±3467)reads,HCV感染者为(17611±5632)reads,也无显著差异(P>0.05);本组228例(97.9%)HCV感染者为单一HCV基因型感染,HIV/HCV混合感染者HCV 6a型和3b型感染率显著高于HCV感染者(分别为47.4%对26.8%,P=0.001和13.7%对3.6%,P=0.005),HCV 1b型感染率显著低于HCV感染者(20.0%对59.4%,P<0.001);在233例入组的HCV感染者中,发现5例(2.1%)为混合基因型感染,其中4例为HIV/HCV混合感染者,1例为HCV感染者。结论广州地区HIV/HCV混合感染者与HCV感染者感染HCV基因型构成比存在差异,其临床意义还需要探讨。Objective To investigate the distribution of HCV genotypes between patients with HIV/HCV co-infection and with HCV mono-infection in Guangzhou.Methods A total of 233 patients with chronic hepatitis C were enrolled in this study,including 95 patients with HIV/HCV co-infection and 138 patients with HCV mono-infection.The HCV core and NS5B gene fragments were used for genotyping by using Illumina Miseq platform PE300 model,and for the samples which the NGS data contained mixed HCV infection(more than 1%reads mapping to other genotypes),the NS5A genotypes specific primers were used to validate the mixed HCV infection.The data was compared by x2 test,fisher’s exact test,t-Student and Mann-Whitney U test.Results The percentage of intravenous drug users(IDUs)in patients with HIV/HCV co-infection was significantly higher than in patients with HCV mono-infection(77.7%vs.19.6%,P<0.001),and the percentage of blood transfusion history in patients with HCV mono-infection was much higher than in those with HIV/HCV co-infection(48.6%vs.3.2%,P<0.001);the average reads of HCV core gene in patients with HIV/HCV co-infection were(15456±6689)reads,no significantly different as compared to(14323±5321)reads in patients with HCV mono-infection,and th e average reads of HCV NS5B gene in patients with HIV/HCV co-infection were(16432±3467)reads,also no significantly different compared to(17611±5632)reads in patients with HCV mono-infection(P>0.05);228 patients(97.9%)in our series were infected with single HCV genotypes,and HCV genotype 6a and 3b infections were more prevalent in patients with HIV/HCV co-infection than in with HCV mono-infection(47.4%vs.26.8%,P=0.001 and 13.7%vs.3.6%,respectively,P=0.005)and HCV genotype 1b infection was more prevalent in patients with HCV mono-infection than in patients with HIV/HCV co-infection(59.4%vs.20.0%,P<0.001);5 patients(2.1%)were found with mix HCV genotype infection,including 4 patients with HIV/HCV co-infection and 1 patient with HCV mono-infection.Conclusion The prevalence of infected HC

关 键 词：慢性丙型肝炎 艾滋病 HCV基因型 二代测序 

分 类 号：R51[医药卫生—内科学]