奶水牛新型可视孕检技术的应用  

Application of New Visual Pregnancy Test Technology in Dairy Buffalo

作　　者：陈明棠[1] 李辉[1] 黄健[1] 谭正准[1] 赵朝步[1] 钟华配 曾咏芳 CHEN Ming-tang;LI Hui;HUANG Jian(Guangxi Buffalo Research Institute,Chinese Academy of Agricultural Sciences,Guangxi Key Laboratory of Buffalo Genetics,Breeding and Reproduction,Ministry of Agriculture and Rural Affairs,Nanning,Guangxi 530001)

机构地区：[1]中国农业科学院广西水牛研究所,农业农村部(广西)水牛遗传繁育重点实验室,广西南宁530001 [2]南宁市阮升贸易有限公司,广西南宁530021

出　　处：《安徽农业科学》2019年第17期98-99,共2页Journal of Anhui Agricultural Sciences

基　　金：广西水产畜牧业科技项目[桂渔牧科(201633012)];南宁市科学研究与技术开发计划项目(南科重20172134-1);广西科技计划项目(桂科AB16380040)

摘　　要：为了缩短奶水牛的产犊间隔,提高繁殖效率和养殖效益,利用酶联免疫反应原理采用一种快速可视孕检试剂盒对配种后28d以上的试验母牛进行检测。对429例已配种母牛进行检测,其中阴性(空怀)265例,阳性(怀孕)164例,验证检查阴性265例,阳性160例,试剂盒检测的阴性准确率为100%,阳性准确率为97.56%,可见该试剂盒可用于水牛的早期孕检。In order to shorten the calving interval of dairy buffalo and improve the efficiency of breeding and raising efficiency,we used enzyme-linked immune reaction principle,adopted a quick and visual pregnancy test kit to make early pregnancy test on experimental cows more than 28 days after mating.429 cases of mated cows were detected,the results showed that 265 cows were negative(empty),164 cows were positive(pregnant),and 265 cows were negative by validation check and 160 cows were positive by validation check.The negative accuracy was 100%and the positive accuracy was 97.56%by ELISA kit.Therefore,this kit was suitable for early pregnancy test of buffalo.

关 键 词：奶水牛 早期孕检 酶联免疫反应试剂盒 产犊间隔 

分 类 号：S823.3[农业科学—畜牧学]