PGPR菌剂对植烟土壤微生物群落结构的影响  

Effects of PGPR on the Microbial Community Structure of Tobacco-growing Soil

作　　者：冯文龙 辜运富[2] 余伟[1] 闫芳芳[1] 张映杰 张宗锦[1] 罗富国[1] 白加林[1] 周欢 FENG Wen-long;GU Yun-fu;YU Wei(Panzhihua Branch of Sichuan Tobacco Corporation,Panzhihua,Sichuan 617099;College of Resources,Sichuan Auricultural University,Chengdu,Sichuan 611130)

机构地区：[1]四川省烟草公司攀枝花市公司,四川攀枝花617099 [2]四川农业大学资源环境学院,四川成都611130

出　　处：《安徽农业科学》2019年第17期146-150,共5页Journal of Anhui Agricultural Sciences

摘　　要：采用PCR-DGGE技术对烟草土壤中细菌群落结构进行分析,对其中10条优势条带进行切胶回收、克隆测序及系统发育分析。DGGE图谱结果显示,施用多功能菌剂后细菌群落结构多样性比未施用多功能菌剂高。在采用多功能菌剂的前提下,减少化肥的施用量不会对烤烟的农艺性状和理化性质造成不良影响,T3处理(减肥10%)对土壤和烤烟的影响最明显。典范对应分析(canonical correspondence analysis,CCA)显示,pH、全氮和有效磷是造成烤烟土壤微生物群落结构变化的主要因素。PCR-DGGE(polymerase chain reaction-denaturing gradient gel electrophoresis)technology was employed to analyze the composition of bacterial community in selective different soil samples.The recovery,cloning,sequencing and phylogenetic analysis of 10 dominant bands were performed.The results of the DGGE showed that there was a difference in the diversity of bacteria between the soil samples with and without PGPR.Under the premise of use of microbial inoculants,reducing the use of fertilizers would not adverse effects on the agronomic traits and physicochemical properties of flue-cured tobacco.T3 treatment was most obvious impact on the soil and flue-cured tobacco.The canonical correspondence analysis(CCA)showed that soil pH,TN and A-P were the critical factors to shape the bacterial community structure in tobacco-growing soil.

关 键 词：PGPR菌剂 植烟土壤 DGGE 群落结构 

分 类 号：S154.3[农业科学—土壤学]