流式细胞外周血刺激法检测Th1/Th2在复发性流产中变化探讨  

The study of the change of Th1/Th2 value of women with recurrent spontaneous abortion detected by flow cytometry peripheral blood stimulation method

作　　者：苗竹林[1] 钟兴明[1] 崔蓉[1] 黄密琼[1] 钟文瑶 王永霞[1] 赵文忠[1] 张碧云[1] MIAO Zhulin;ZHONG Xingmin;CUI Rong;HUANG Miqiong;ZHONG Wenyao;WANG Yongxia;ZHAO Wenzhong;ZHANG Biyun(Family Planning Research Institute of Guangdong Province,Guangzhou Province,510600)

机构地区：[1]广东省计划生育专科医院广东省计划生育科学技术研究所

出　　处：《中国计划生育学杂志》2019年第8期1044-1047,共4页Chinese Journal of Family Planning

基　　金：广东省自然科学基金(2016A030313760);广州市科技计划项目(201510010253;1563000217)

摘　　要：目的:探讨流式细胞外周血刺激法检测Th1、Th2及Th1/Th2在复发性流产(RSA)中变化。方法:2012年1月—2018年5月本院就诊患者,妊娠前有复发性自然流产病史310例为RSA组,无自然流产病史220例为对照组;早孕有复发性流产史140例为早孕RSA组,正常早孕102例为早孕对照组;稽留流产患者19例。经过外周血PMA+Ion刺激后采用流式细胞技术检测Th1(CD4+/IFN-γ+)和Th2细胞(CD4+/IL-4+),计算Th1/Th2值。结果:非妊娠期,RSA组与对照组均处于Th1优势状态,未发生向Th2偏移,两组Th1/Th2值比较未见差异(P>0.05);早孕RSA组与早孕对照组比较仍处于Th1优势状态,未发生向Th2偏移,两组Th1/Th2值比较未见差异(P>0.05);稽留流产患者为Th1优势状态,未发生向Th2偏移。结论:复发性流产患者妊娠前后、稽留流产患者均处于Th1优势状态,均未发生Th1/Th2失衡。Objective:To investigate the change of Th1/Th2 value of women with recurrent spontaneous abortion(RSA)detected by flow cytometry peripheral blood stimulation method.Methods:310 women with RSA were included in group A,220 women without history of spontaneous abortion were in group B,140 pregnant women with RSA were in group C,and 102 normal pregnant women were group D.There were 19 pregnant women with missed abortion.The levels of Th1 cells(CD4+/IFNγ+)and Th2 cells(CD4+/IL 4+)of all included women were detected by flow cytometry peripheral blood stimulation method after peripheral blood PMA+Ion stimulation,and the value of Th1/Th2 was calculated.Results:The Th1 cells levels of women in group A and the group B were always in the dominant state,and the dominant had no deviation to Th2 cell,there was no significant different in the value of Th1/Th2 of women between group A and group B(P>0.05).The Th1 cells levels of women in group C and the group D were still in the dominant state,and the dominant had no deviation to Th2 cell,there was no significant different in the value of Th1/Th2 of women between group C and group D(P>0.05).The Th1 cells level of pregnant women with missed abortion was also in the dominant state,and the dominant had no deviation to Th2 cell.Conclusion:The Th1 cells level of women with RSA before or after pregnancy and of the pregnant women with missed abortion are always in the dominant state,and the dominant has never deviation to their Th2 cell.

关 键 词：复发性自然流产 稽留流产 细胞因子 TH1/TH2 流式细胞技术 

分 类 号：R71[医药卫生—妇产科学]