IL1RN基因在晋汾白猪仔猪和新山西黑猪仔猪不同组织和细胞中的表达特征分析  

Characterization of Cellular and Tissular Expression of IL1RN Gene in Jinfen White Piglets and New Shanxi Black Piglets

作　　者：孙奴奴 马晶晶 SUN Nu-nu;MA Jing-jing(Department ofLife Science,Yuncheng University,Yuncheng 044000,China)

机构地区：[1]运城学院生命科学系

出　　处：《畜牧与饲料科学》2019年第8期1-5,共5页Animal Husbandry and Feed Science

基　　金：山西省重点学科建设经费资助（FSKSC）;山西省“1331”工程重点学科项目（098-091704）

摘　　要：白细胞介素1受体拮抗剂(interleukin 1 receptor antagonist,IL1RN)是一种重要的宿主免疫调节因子,是天然存在的IL-1拮抗剂。为了解IL1RN基因在断奶仔猪不同组织和细胞中表达的特征,利用Trizol法提取晋汾白猪仔猪和新山西黑猪仔猪的心脏、肝脏、脾脏、淋巴细胞、巨噬细胞等18种组织和细胞的总RNA,采用RT-PCR方法检测IL1RN基因在不同组织和细胞中的表达情况,应用SPSS 19.0软件分析IL1RN基因在晋汾白猪仔猪和新山西黑猪仔猪4种免疫组织和细胞(脾脏、淋巴结、巨噬细胞、白细胞)中的表达差异情况。结果显示:IL1RN基因在晋汾白猪仔猪和新山西黑猪仔猪的脂肪组织中均不表达,在其余各组织和细胞中均表达;IL1RN基因在晋汾白猪仔猪的巨噬细胞与淋巴结、脾脏、白细胞之间的表达差异极显著(P<0.01),且在淋巴结与脾脏、白细胞之间的表达差异极显著(P<0.01);IL1RN基因在新山西黑猪仔猪的脾脏、淋巴结、巨噬细胞、白细胞的表达两两之间均呈极显著差异(P<0.01);IL1RN基因在2个猪种的脾脏、巨噬细胞、白细胞之间的表达呈极显著差异(P<0.01),在淋巴结中的表达呈显著差异(P<0.05)。该研究结果为进一步揭示2个猪种的IL1RN基因在疾病抵抗方面的作用及相关机制提供了依据。Interleukin 1 receptor antagonist(IL1RN)is an important host immunoregulatory factor and is a natural IL-1 antagonist.This study aimed to characterize the cellular and tissular expression of IL1RN gene in weaned piglets of two local pig breeds in Shanxi Province.The total RNA of 18 tissues and cells,including heart,liver,spleen,lymphocytes,macrophages,etc.,of Jinfen white piglets and new Shanxi black piglets was extracted by Trizol method.The expression of IL1RN gene in different tissues and cells of the piglets was determined by RT-PCR assay.The expressional difference of IL1RN gene in four immune related tissues and cells(spleen,lymph nodes,macrophages,white blood cells)between the two local pig breeds were statistically compared with SPSS 19.0 software.The results showed that except for adipose tissue,the expression of IL1RN gene was observed in all the other tested tissues and cells in both of the two local pig breeds;extremely significant(P<0.01)expressional difference in IL1RN gene between macrophages and lymph nodes,spleen,white blood cells in Jinfen white piglets was found,and that between lymph nodes and spleen,white blood cells were also observed;the expressional difference in IL1RN gene between spleen,lymph nodes,macrophages and white blood cells in new Shanxi black piglets was extremely significant(P<0.01)when compared pairwisely;there were extremely significant(P<0.01)expressional differences in IL1RN gene in spleen,macrophages and white blood cells between the two local pig breeds,and significant(P<0.05)expressional difference in IL1RN gene in lymph nodes between the two local pig breeds was found.Our results provide a basis for further unraveling the function and underling mechanism of the IL1RN gene in disease resistance in the two local pig breeds.

关 键 词：IL1RN基因 晋汾白猪 新山西黑猪 仔猪 表达差异 RT-PCR 

分 类 号：S828.1[农业科学—畜牧学]