绵羊卵母细胞不同cAMP水平模型的构建  

Construction of Ovine Oocytes Model with Different Intracellular cAMP Levels

作　　者：赵玉芬 娜美尔格 杨炳雪 白玥 于泊洋 李海军[1,2] ZHAO Yu-fen;NAMEI Erge;YANG Bing-xue;BAI Yue;YU Bo-Yang;LI Hai-jun(College of Veteririary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Key Laboratory of Basic Veterinary Science,Hohhot 010018,China;Basic Medical College,inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]内蒙古自治区基础兽医学重点实验室,呼和浩特010018 [3]内蒙古医科大学基础医学院,呼和浩特010110

出　　处：《内蒙古大学学报（自然科学版）》2019年第4期346-352,共7页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金(31760717)

摘　　要：环腺苷酸(cAMP)是维持哺乳动物卵母细胞减数分裂停滞的关键分子.本试验利用ELISA和地衣红染色技术,检测了西洛酰胺(Cilostamide)与乌药醚内酯(Linderane)对绵羊卵母细胞和卵丘细胞内cAMP水平以及生发泡破裂(GVBD)的影响.结果显示:体外培养3h后,10μmol/L Cilostamide和5μmol/L Linderane分别能显著提升和抑制卵母细胞而非卵丘细胞内的cAMP水平;体外培养6h后,Cilostamide组卵母细胞GVBD率(14.91%)显著低于对照组(34.45%)与联合添加组(33.47%)(P<0.05),而Linderane组与其相反,表明不同cAMP水平卵母细胞模型构建成功.cAMP is a key molecule in maintaining mammalian oocyte meiosis arrest.In this study,ELISA and orcein staining techniques were chosen to detect the effects of Cilostamide and Linderane on intracellular cAMP levels in ovine oocytes and cumulus cells and the ratio of oocyte germinal vesicle break down(GVBD),respectively.Our results showed thatCilostamide(10 μmol/L)and Linderane(5 μmol/L)significantly increased and inhibited intra-oocyte cAMP level rather than those in cumulus cells after 3h of IVM.In addition,The GVBD rates of oocytes in Cilostamide group(14.91%)was significantly lower than those of the control(34.45%)and combined group(33.47%)after cultured in vitro for 6h(P<0.05),andwas the completely opposite of those in Linderane group,indicating that the ovine oocyte model with different cAMP levels has been constructed successfully.

关 键 词：绵羊 卵母细胞 环腺苷酸 西洛酰胺 乌药醚内酯 生发泡破裂 

分 类 号：S826.3[农业科学—畜牧学] S852.1[农业科学—畜牧兽医]