生长因子对猪卵母细胞成熟及孤雌胚胎发育的影响  

Effects of Growth Factors on in vitro Maturation of Porcine Oocytes and Preimplantation Development of Parthenogenetic Embryos

作　　者：张曼玲[1,2] 金永 赵丽华[1] 王晨宇[1] 刘曼菱 张红 尤志欢[1] 李荣凤 ZHANG Man-ling;JIN Yong;ZHAO Li-hua;WANG Chen-yu;LIU Man-ling;ZHANG Hong;YOU Zhi-huan;LI Rong-feng(Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University,Nanjing 211166,China;Research Center for Animal Genetic Resources of Mongolian Plateau,Inner Mongolia University,Hohhot 010070,China)

机构地区：[1]南京医科大学江苏省异种移植重点实验室,南京211166 [2]内蒙古大学生命科学学院蒙古高原动物遗传资源研究中心,呼和浩特010070

出　　处：《内蒙古大学学报（自然科学版）》2019年第4期397-403,共7页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金项目(31371487)

摘　　要：主要探讨了三种生长因子对猪卵母细胞成熟及孤雌胚胎发育及多能基因表达的影响,结果表明:成熟液中添加生长因子后可显著提高卵母细胞成熟率;成熟液和发育液中都添加生长因子后胚胎卵裂率、囊胚率及胚胎质量均显著升高;添加生长因子可极显著提高孤雌囊胚中多能基因的表达.本研究为提高猪卵母细胞成熟及胚胎早期发育奠定了基础,进而为获得更利于分离培养猪胚胎干细胞系的囊胚材料提供了参考.The effects of three growth factors on the maturation of porcine oocytes,the development and pluripotent genes expression of porcine early parthenogenetic embryos were analyzed in this study.The results showed that when the growth factors were supplemented in the oocyte in vitro maturation media,the maturation rate could be significantly increased.When the growth factors were supplemented in both in vitro maturation media and porcine zygotes medium 3(PZM-3),the cleavage rate and blastocyst rate,as well as the quality of the parthenogenetic embryos were significantly increased.Moreover,the growth factors could significantly improve the expression level of pluripotent genes OCT4 and SOX2 in parthenogenetic blastocysts.This study provided a useful reference in improving the maturation of porcine oocytes and the early embryonic development,and will be also helpful for isolation and establishment of porcine embryonic stem cell lines.

关 键 词：生长因子 猪 卵母细胞 孤雌发育 

分 类 号：S814.6[农业科学—畜牧学] Q95-336[农业科学—畜牧兽医]