基于多元统计分析的麸炒白术质量评价研究  

Quality evaluation of bran-processed Atractylodes Macrocephala Rhizoma based on multivariate statistical analysis

作　　者：赵丽沙 董宇[1] 李功华[2] 俞忠明[1] 寿旦[1] ZHAO Li-sha;DONG Yu;LI Gong-hua;YU Zhong-ming;SHOU Dan(Department of Pharmacy,Zhejiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China;Department of Pharmacy,Tongde Hospital of Zhejaing Province,Hangzhou 310012,China)

机构地区：[1]浙江省中医药研究院中药研究中心,杭州310007 [2]浙江省立同德医院药学部,杭州310012

出　　处：《药物分析杂志》2019年第8期1443-1449,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：国家中药标准化项目(ZYBZH-Y-ZY-45);浙江省中医药科技计划项目(2016ZB008,2018ZY003);浙江省基础公益研究计划(LGC19H280006)

摘　　要：目的:对麸炒白术饮片中浸出物、挥发油及指标性成分的含量进行多元统计分析,明确麸炒白术饮片质量的显著影响因素,为白术饮片质量评价提供依据。方法:采用高效液相色谱法测定麸炒白术饮片中白术内酯Ⅰ、白术内酯Ⅱ和白术内酯Ⅲ的含量,以2015年版《中华人民共和国药典》浸出物测定法测定醇溶性浸出物的含量,挥发油测定法甲法测定挥发油的含量。以白术内酯Ⅰ、白术内酯Ⅱ和白术内酯Ⅲ、白术总内酯、浸出物、挥发油为指标,采用SPASS19.0软件对38批麸炒白术饮片进行聚类分析,采用SIMCA-P13.0软件对38批麸炒白术饮片进行主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)。结果:以白术内酯Ⅰ、白术内酯Ⅱ和白术内酯Ⅲ、浸出物、挥发油为指标,采用多元统计分析,结果表明白术内酯Ⅱ是影响麸炒白术质量的显著性因素。结论:建立的HPLC方法可用于麸炒白术饮片中3个指标性成分的含量测定,为后续白术饮片等级划分提供依据。Objective:Multivariate statistical analysis was carried out on the contents of atractylenolideⅠ,atractylenolideⅡ and atractylenolideⅢ,extract and volatile oil to clarify the significant factors affecting the quality of the bran-processed Atractylodes Macrocephala Rhizoma,and provide scientific basis for quality evaluation. Methods:The contents of atractylenolideⅠ,atractylenolideⅡ and atractylenolideⅢ were determined by high performance liquid chromatography,and the contents of alcohol soluble extract and volatile oil were determined referring to the Pharmacopoeia of The People’s Republic of China. Using contents of atractylenolideⅠ,atractylenolide Ⅱ and atractylenolide Ⅲ,the total content of atractylenolide,extract and volatile oil as indicators,SPASS19.0 software was used to cluster 38 batches of bran-processed Atractylodes Macrocephala Rhizoma. SIMCA-P13.0 software was used for principal component analysis(PCA)and partial least squares discriminant analysis(OPLS-DA). Results:Using atractylenolideⅠ,atractylenolide Ⅱ and atractylenolide Ⅲ,extract and volatile oil as indicators,multivariate statistical analysis was used for data analysis. The results showed that atractylenolide Ⅱ was a significant factor affecting the quality of bran-processed Atractylodes Macrocephala Rhizoma. Conclusion:The established HPLC method can be used to determine the contents of three index components in the bran-processed Atractylodes Macrocephala Rhizoma,which will provide a basis for the subsequent classification of bran-processed Atractylodes Macrocephala Rhizoma.

关 键 词：麸炒白术 白术内酯 浸出物 挥发油 多元统计分析 质量评价 

分 类 号：R917[医药卫生—药物分析学]