瑞芬太尼对大鼠脊髓背角神经元铁代谢的影响  

Effect of remifentanil on iron metabolism in spinal dorsal horn neurons of rats

作　　者：舒瑞辰 李媛 张霄蓓 王国林[2] 赵洪伟 Shu Ruichen;Li Yuan;Zhang Xiaobei;Wang Guolin;Zhao Hongwei(Department of Anesthesiology,Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin′s Clinical Research Center for Cancer,Tianjin 300060,China;Department of Anesthesiology,Tianjin Medical University General Hospital,Tianjin 300052,China)

机构地区：[1]天津医科大学肿瘤医院麻醉科,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,天津市恶性肿瘤临床医学研究中心,300060 [2]天津医科大学总医院麻醉科,300052

出　　处：《中华麻醉学杂志》2019年第5期582-585,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81371245);天津市教委科研计划项目(2018KJ066)

摘　　要：目的评价瑞芬太尼对大鼠脊髓背角神经元铁代谢的影响。方法培养大鼠原代脊髓背角神经元,以2×10^5个/孔的密度接种培养孔,采用随机数字表法分为4组:对照组(C组,n=40)、瑞芬太尼组(R组,n=40)、铁反应元件阴性二价金属离子转运体1[DMT1(-)IRE]siRNA组(siRNA组,n=32)和DMT1(-)IRE siRNA+瑞芬太尼组(siRNA+R组,n=32)。siRNA组和siRNA+R组于培养第3天进行DMT1(-)IRE siRNA转染。R组和siRNA+R组在终浓度40 nmol/L瑞芬太尼溶液中孵育60 min。R组和siRNA+R组于瑞芬太尼孵育结束时、其余组于相应时点采用荧光探针法测定ROS和Fe^2+含量,采用TBA法测定MDA含量,采用calcein AM和铁螯合剂检测细胞可变铁池(LIP)含量,R组和C组采用Western blot法测定DMT1(-)IRE和DMT1(+)IRE的表达。结果与C组比较,R组脊髓背角神经元DMT1(-)IRE表达上调,Fe^2+、LIP、ROS和MDA含量升高(P<0.05),DMT1(+)IRE表达差异无统计学意义(P>0.05);与R组比较,siRNA+R组脊髓背角神经元Fe^2+、LIP、ROS和MDA含量降低(P<0.05)。结论瑞芬太尼通过激活DMT1(-)IRE,引起脊髓背角神经元铁含量增加,该过程可能与瑞芬太尼诱发大鼠术后痛觉过敏的机制有关。Objective To evaluate the effect of remifentanil on iron metabolism in spinal dorsal horn neurons of rats.Methods The primary spinal dorsal horn neurons of rats were seeded in the culture plate at a density of 2×10^5 cells/well and divided into 4 groups using a random number table method:control group(C group,n=40),remifentanil group(R group,n=40),divalent metal transporter 1 without iron-responsive element[DMT1(-)IRE]siRNA group(siRNA group,n=32)and DMT1(-)IRE siRNA plus remifentanil group(siRNA+R group,n=32).siRNA and siRNA+R groups were subjected to DMT1(-)IRE siRNA transfection on day 3 of culture.R and siRNA+R groups were incubated for 60 min in the solution with remifentanil at a final concentration of 40 nmol/L.The contents of reactive oxygen species(ROS)and Fe2+were determined by fluorescent probe method,the malondialdehyde(MDA)content was detected by TBA method,and the content of labile iron pool(LIP)was detected by calcein AM and iron chelator at the end of incubation with remifentanil in R and siRNA+R groups and at the corresponding time points in the other groups.The expression of DMT1(-)IRE and DMT1(+)IRE was determined by Western blot in C and R groups.Results Compared with C group,the expression of DMT1(-)IRE in the spinal dorsal horn neurons was significantly up-regulated,the contents of Fe^2+,LIP,ROS and MDA were increased(P<0.05),and no significant change was found in the expression of DMT1(+)IRE in R group(P>0.05).Compared with R group,the contents of Fe^2+,LIP,ROS and MDA in the spinal dorsal horn neurons were significantly decreased in siRNA+R group(P<0.05).Conclusion Remifentanil increases the iron content of spinal dorsal horn neurons by activating DMT1(-)IRE,which may be associated with the mechanism of remifentanil-induced postoperative hyperalgesia in rats.

关 键 词：哌啶类 痛觉过敏 铁 脊髓 

分 类 号：R61[医药卫生—外科学]