厦门地区腹泻儿童A组轮状病毒基因型别流行特征  

Molecular epidemiology of group A rotavirus in children with diarrhea in Xiamen

作　　者：王舜钦 林建成 肖秀香 吴海明 徐锦 Wang Shunqin;Lin Jiancheng;Xiao Xiuxiang;Wu Haiming;Xu Jin(Department of Clinic Laboratory,Xiamen Children′s Hospital,Xiamen Key Laboratory of Neonatal Diseases,Xiamen 361006,China;Clinical Laboratory Center,Children′s Hospital of Fudan University,Shanghai 201102,China)

机构地区：[1]厦门市儿童医院医学检验科厦门市新生儿疾病重点实验室,361006 [2]复旦大学附属儿科医院临床检验中心,上海201102

出　　处：《中华传染病杂志》2019年第6期327-331,共5页Chinese Journal of Infectious Diseases

基　　金：厦门市科技惠民计划项目(3502Z20164072)

摘　　要：目的监测厦门地区岛内外4家不同医院门诊和住院腹泻患儿A组轮状病毒感染基因型特征,为防治轮状病毒感染所致腹泻提供基础数据和理论依据。方法对2016年1月至2017年12月厦门地区4家医院共5 787例年龄≤10岁腹泻患儿粪便样本进行免疫层析双抗体夹心法检测,按检测时间先后顺序对1 435例轮状病毒抗原阳性标本进行编号,再依序等间隔抽取98份轮状病毒阳性样本,采用反转录套式聚合酶链式反应对样本进行A组轮状病毒G基因型及P基因型分型检测。结果5 787例粪便标本中A组轮状病毒抗原阳性1 435例,检出率为24.8%。98份轮状病毒阳性样本进行基因分型G分型结果显示,G9型有68株(69.4%)为主要流行株,其次是G2型5株(5.1%),G1型4株(4.1%),G3型1株(1.0%),G未能分型20株;P分型P[8]型74株(75.5%)为主要流行株,P[4]型5株(5.1%),P未能分型19株。G/P组合基因型P[8]G9型检出63株(64.3%)为主要基因组合型,其次是P[4]G2型5株(5.1%),P[8]G1型4株(4.1%),P[8]G3型1株(1.0%)。结论轮状病毒是引起该地区患儿腹泻的主要病原体之一,P[8]G9型为主要基因组合型,持续监测A组轮状病毒基因型对该地区A组轮状病毒感染防控及疫苗准入是必要的。Objective To investigate the epidemiological characteristics and genotypes of group A rotavirus(RV-A)among inpatients and outpatients children with diarrhea in Xiamen to provide basic data and theoretical basis for prevention and treatment of rotavirus diarrhea.Methods A total of 5 787 fecal samples from children under 10 years old in four hospitals in Xiamen from Jan 2016 to Dec 2017 were detected by immunochromamatoraphy double antibody sandwich assay.Systematic sampling was applied for collection of 98 fecal samples from 1 435 samples with rotavirus positive.Reverse transcription nested PCR was applied for determination of G and P genotypes.Results Among the 5 787 patients,1 435 specimens were detected to be RV positive(24.8%).Genotyping of 98 rotaviruses showed that G9(69.4%)was the most predominant,followed by G2(5.1%),G1(4.1%)and G3(1.0%).Twenty cases were undetermined as G type.For P types,P[8]was predominant,accounting for 75.5%and the prevalence of P[4]was 5.1%.Nineteen cases were undetermined as P type.The combination of genotypes were P[8]G9(64.3%),followed by P[4]G2(5.1%),P[8]G1(4.1%)and P[8]G3(1.0%).Conclusions Rotavirus is the main pathogen among infants and children with diarrhea in Xiamen.P[8]G9 is the most prevalent genotypes.Continuously monitoring RV-A epidemic genotypes is helpful to provide data for local prevention and control of RV-A infection and introduction of rotavirus vaccine.

关 键 词：轮状病毒属 腹泻 基因型 流行病学 

分 类 号：R72[医药卫生—儿科]