高糖高脂饲料诱导的小型猪肥胖模型  

High Fat and High Sugar Diet Induced the Fat Model of Bama Miniature Pigs

作　　者：相磊 陈华[2] 赵德明[1] XIANG Lei;CHEN Hua;ZHAO Deming(College of veterinary medicine,china agricultural university,Beijing 100093,China;Laboratory animal center of PLA general hospital,Beijing 100853,China)

机构地区：[1]中国农业大学动物医学院,北京100093 [2]中国人民解放军总医院医学实验动物中心,北京100853

出　　处：《实验动物科学》2019年第4期1-5,9,共6页Laboratory Animal Science

基　　金：国家自然科学基金(No.31472057)

摘　　要：目的探讨高脂高糖饲料对巴马小型猪脂肪分布与血液生化指标的影响。方法巴马小型猪14头,实验组8头,为本实验室培育的2型糖尿病易感小型猪,饲喂高脂高糖饲料30周;对照组6头饲喂正常饲料。30周后测定体型系数、CT扫描脂肪含量、采集血液检测生化指标,比较实验组与对照组的差异。结果高脂高糖饲料饲喂30周后实验组动物与对照组动物在体质量、体质指数(BMI)、脂肪总含量差异显著(P<0.05),内脏脂肪含量差异极显著(P<0.01);血液生化指标中胰岛素、总胆固醇、高密度脂蛋白胆固醇、甘油三脂与对照组相比差异极为显著(P<0.01),低密脂蛋白胆固醇与对照组相比差异显著(P<0.05)。此外,实验组巴马小型猪血清蛋白、肌酸酐与对照组差异显著(P<0.05);尿酸、尿素与对照组差异极显著(P<0.01),显示实验组小型猪呈现肾损伤。结论高脂高糖饮食能够引起巴马小型猪脂肪堆积,内脏脂肪堆积尤为明显,血液生化指标呈高脂血症伴有肾功能的损伤。Objective The effects of high fat and high sugar diet(HFHSD)on fat distribution and blood biochemical parameters in Bama miniature pigs.Method Fourteen Bama minipigs were divided into two groups.Experimental group pigs fed with high fat and high sugar diet for 30 weeks.Six Bama miniature pigs in control group were fed with normal feed.After 30 weeks,body mass index,fat distribution and biochemical indexes of blood were measured.Result The body weight,body mass index(BMI)and total fat content especially visceral fat content increased significantly in experimental group.Blood biochemical indicators of insulin,total cholesterol,high and low density lipoprotein cholesterol,blood creatinine,uric acid and urea were increased significantly compared with control group.Conclusion High fat and high sugar diet can cause fat accumulation in Bama miniature pigs,especially visceral fat.Blood biochemistry is characterized by hyperlipidemia and impaired renal function.

关 键 词：巴马小型猪 脂肪分布 血液生化 2型糖尿病 

分 类 号：Q493[生物学—生理学]