不同时期绿色荧光裸鼠雌鼠血浆及组织中性激素水平变化  

The Changes of Plasma and Tissue Levels of Sex Hormones in Nude Female Mice with Green Fluorescence at Different Stages

作　　者：尤金炜 董敏 刘彪 赵志刚 王丽娴 马畅 张旭亮 刘捷 恽时锋 YOU Jinwei;DONG Min;LIU Biao;ZHAO Zhigang;WANG Lixian;MA Chang;ZHANG Xuliang;LIU Jie;YUN Shifeng(Department of Comparative Medicine,General Hospital of Eastern Theater Command,Army Education Base of Science and Ethics of Experimental Animal,National Sciene Education Base,Nanjing 210002,China;College of Food Science and Technology of Nanjing Agricultural University 210002,China)

机构地区：[1]东部战区总医院比较医学科,全军实验动物科普与伦理教育基地,全国科普教育基地,南京210002 [2]南京农业大学食品科技学院,南京210002

出　　处：《实验动物科学》2019年第4期10-13,共4页Laboratory Animal Science

基　　金：中国人民解放军东部战区总医院科学研究课题(No.2016056)

摘　　要：目的了解不同妊娠期、泌乳期绿色荧光(Green Fluorescence Protein,简称GFP)裸鼠雌鼠血浆中雌激素、孕激素、泌乳素及各组织中泌乳素及泌乳素受体mRNA水平变化。方法通过电化学发光法测定血浆中激素的水平,通过荧光定量PCR法测定泌乳素及泌乳素受体mRNA相对表达量。结果GFP裸鼠雌鼠妊娠期内雌激素和泌乳素水平不断下降、孕激素的水平先升后缓慢下降、组织中泌乳素mRNA水平较低、泌乳素受体水平较高;而在泌乳期内雌激素、孕激素水平较稳定、组织中泌乳素mRNA水平较高,泌乳素受体水平较低。结论不同时期GFP裸鼠雌鼠体内性激素的水平不同,参与裸鼠妊娠调控过程,为解决雌鼠不孕、易流产等问题提供了基础参考值和内分泌水平的解决思路。To understand the changes in plasma levels of estrogen,progesterone,prolactin,and prolactin receptor mRNA in mice during pregnancy and lactation.Plasma levels of progesterone were measured by electro-chemiluminescence and relative mRNA levels of prolactin and prolactin receptor were measured by fluorescence quantitative PCR.During the gestation period,estrogen and prolactin levels decreased continuously,progesterone levels increased first and then decreased slowly,prolactin mRNA levels were low in tissues,and prolactin receptor levels were high.During lactation,the levels of estrogen and progesterone are relatively stable,the levels of prolactin mRNA in tissues are relatively high,and the levels of prolactin receptors are relatively low.Different levels of sex hormones in nude mice with green fluorescence were different in different periods,and they participated in the process of pregnancy regulation in nude mice.It provides basic reference value and endocrine level for solving the problems of female mouse infertility and abortion.

关 键 词：绿色荧光裸鼠 雌激素 孕激素 泌乳素 泌乳素受体 

分 类 号：Q956[生物学—动物学]