NHEJ通路相关基因在电离辐射所致脑损伤中的表达情况研究  

Research of expression of NHEJ pathway related genes in brain injury induced by ionizing radiation

作　　者：李梅[1] 刘梦雅[1] 王新钢[1] 黄立群[1] 孙鸽[1] 尹晶晶[1] 秦秀军[1] LI Mei;LIU Mengya;WANG Xingang;HUANG Liqun;SUN Ge;YIN Jingjing;QIN Xiujun(Department of Radiological and Evironmental Medicine,China Institute for Radiation Protection,Taiyuan 030006 China)

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所

出　　处：《中国辐射卫生》2019年第3期223-227,共5页Chinese Journal of Radiological Health

基　　金：山西省自然科学基金(201801D121363);山西省科技基础条件平台项目(201805D141007)

摘　　要：目的通过对大鼠头部进行不同剂量的照射,研究NHEJ通路相关基因在电离辐射所致脑损伤中的表达情况。方法利用医用电子加速器装置对SD大鼠分别进行10 Gy、20 Gy、30 Gy的照射,并于照后30天提取动物海马组织中的总RNA;30 Gy照射组在照后6 h、24 h、72 h、7天、30天五个不同时间点提取动物海马组织中的总RNA。使用实时荧光定量PCR技术对NHEJ通路中的XRCC4、XRCC5和XRCC6基因的表达情况进行研究。结果经照射大鼠海马组织中与DNA修复相关的基因XRCC4、XRCC5和XRCC6表达均有所上调;20 Gy组XRCC4、XRCC5和XRCC6基因的表达量较10 Gy和30 Gy组高;XRCC4基因在照后7天表达量达到最高,而XRCC5和XRCC6基因在照后6 h达到最高,XRCC4、XRCC5和XRCC6基因表达量均在照后30天达到最低。结论通过本项目研究发现NHEJ通路中与DNA损伤修复有关基因XRCC4、XRCC5、XRCC6在电离辐射所致脑损伤照射不同剂量点及照后不同时间点均上调表达,说明在10、20、30 Gy照射和电离辐射后6 h、24 h、72 h、7天、30天五个不同时间点机体均通过NHEJ通路进行DNA双链修复,且在照后6h和第7天修复能力最强。Objective To study the expression of the genes associated with the NHEJ pathway in brain injury induced by ionizing radiation on the head of rats at different doses.Methods Using medical electron accelerator to irradiate on SD rats at doses of 10Gy,20 Gy and 30 Gy,and the total RNA in animal hippocampus tissue was extracted 30 days after exposure.The RNA of 30Gy irradiation group was extracted in 6 h,24 h,72 h,7 d and 30 d after exposure.The expression of XRCC4,XRCC5 and XRCC6 genes in the NHEJ pathway wasstudied using real-time fluorescence quantitative PCR.Results The expression of genes XRCC4,XRCC5 and XRCC6 associated with DNA repair in irradiated rat hippocampus wasup-regulated.The expression levels of the genes XRCC4,XRCC5 and XRCC6 in the 20 Gy group were higher than those in 10 Gy and 30 Gy groups;The expression of XRCC4 reached the highest level 7 days after exposure,while the expression of XRCC5 and XRCC6 genes reached the highest level 6h after exposure,and the expression levels of XRCC4,XRCC5 and XRCC6 genes reached the lowest level 30 days after exposure.Conclusion The expression of genes XRCC4,XRCC5 and XRCC6 associated with DNA damage repair in the NHEJ pathway at different dose points and at different timepoints after exposure to brain injury caused by radiation was found to be raised.It indicated that the body repaired DNA double strand through NHEJ pathway at 6 h,24 h,72 h,7 days and 30 days after 10,20,30 Gy irradiation and the most powerful repair ability was performed at 6 h and 7 days after exposure.

关 键 词：电离辐射 XRCC4基因 XRCC5基因 XRCC6基因 DNA双链断裂修复 实时荧光定量PCR 

分 类 号：Q691.5[生物学—生物物理学]