齐卡兔瘦蛋白基因在不同组织中的表达水平与肉质指标的关联性  

Correlation between leptin gene expression level in different tissues and meat quality in Zika rabbit

作　　者：罗刚[1] 王杰[1] 陈婷婷[1] 刘林海 赖松家[1] LUO Gang;WANG Jie;CHEN Tingting;LIU Linhai;LAI Songjia(Institute of Animal Genetics and Breeding,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物遗传育种研究所

出　　处：《浙江大学学报（农业与生命科学版）》2019年第4期484-489,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家现代农业产业技术体系建设专项（CARS-44-A-2）;四川省“十三五”畜禽育种攻关项目（2016NYZ0046）

摘　　要：选取齐卡兔(N系)为实验材料,利用荧光定量聚合酶链式反应(quantitative real-time fluorescence polymerase chain reaction,qRT-PCR)技术测定齐卡兔在35、70、84和91日龄4个阶段腿肌、肾周脂肪和皮下脂肪中瘦蛋白基因(leptin)的表达水平,并检测腿肌的pH值、熟肉率、滴水损失、肌内脂肪等相关肉品质指标,从而分析齐卡兔在这3种组织中leptin基因表达水平与所测肉品质指标的关联性。结果表明:母兔肾周脂肪和腿肌中leptin基因的表达量高于公兔,而在皮下脂肪中公兔的leptin基因表达量高于母兔。84日龄母兔皮下脂肪中leptin基因的表达量与肌内脂肪含量、熟肉率和屠宰后24 h的pH值的相关系数分别为0.95(P<0.01)、0.83(P<0.05)和0.67(P<0.05);公兔屠宰后24 h的pH值与腿肌中leptin基因表达量的相关系数为-0.85(P<0.01)。根据分析,认为leptin基因在齐卡兔各组织中的表达量与肉质指标具有显著的相关性,可以作为研究家兔肉质的一个候选基因,从而为采用分子遗传标记技术和开展肉兔的遗传改良提供参考。Zika rabbit(N strain)was selected as the experimental material,and the expression levels of leptin gene(leptin)in leg muscle,perirenal fat and subcutaneous fat at four different days of age(35,70,84,91)of Zika rabbits were determined by quantitative real-time fluorescence polymerase chain reaction(qRT-PCR).The pH value of leg muscle,cooked meat rate,drip loss and intramuscular fat were also measured.The correlation between leptin gene expression level and meat quality index in above the three tissues of Zika rabbits was analyzed.The results showed that leptin gene expression in perirenal fat and leg muscle of female rabbits was higher than that of male rabbits,and the leptin gene expression in subcutaneous fat of male rabbits was higher than that of female rabbits.The correlation coefficients between the expression level of leptin gene in subcutaneous fat and intramuscular fat content,cooked meat rate,pH value of 24 h after slaughter in the 84 dayold female rabbits were 0.95(P<0.01),0.83(P<0.05)and 0.67(P<0.05),respectively;and the correlation coefficient between pH value of 24 h after slaughter and the expression level of leptin gene in the leg muscle in the 84 day-old male rabbits was-0.85(P<0.01).According to the analysis result,the expression level of leptin gene in the tissues of Zika rabbits is significantly correlated with meat quality,and it can be used as a candidate gene for studying meat quality of rabbits,and provide a reference for using molecular genetic marker technology and genetic improvement of meat rabbits.

关 键 词：瘦蛋白基因 齐卡兔 关联性分析 肉质 

分 类 号：Q756[生物学—分子生物学] S829.1[农业科学—畜牧学]