不同结香方法白木香的解剖构造比较  

Anatomy comparative study of Aquilaria sinensis in different agarwood induced methods

作　　者：何海珊[1] 潘质洪[2] 梁永兵 罗蓓[1] 邱坚[1] HE Haishan;PAN Zhihong;LIANG Yongbing;LUO Bei;QIU Jian(College of Material Science and Engineering,Southwest Forestry University,Kumming 650224,China;Zhongshan Polytechnic,Zhongshan 528400,Guangdong,China)

机构地区：[1]西南林业大学材料工程学院,昆明650224 [2]中山职业技术学院,广东中山528400

出　　处：《林业工程学报》2019年第5期54-59,共6页Journal of Forestry Engineering

基　　金：国家自然科学基金(31570555)

摘　　要：白木香(Aquilaria sinensis)为沉香来源树种,在我国亚热带地区作为珍贵树种广泛种植,但因目前人工结香产量及质量不高,沉香产业发展受到极大限制。为研究白木香结香生理过程,开发白木香人工结香技术,对打孔火烙法、剥皮法、虫蛀、化学试剂A法的结香样品进行解剖观察,发现深色次生代谢产物、再生组织、胼胝质、晶体等的形成与分布存在差异。所有方法中仅化学试剂A形成的次生代谢产物沉积(结香)区域呈块状,其余方法形成的结香区呈薄层状或点状或束状。结香区域化学试剂A>伤及木质部的剥皮法/打孔火烙法>新鲜虫蛀>不伤及木质部的环剥树皮法。初步估计化学试剂A的结香量最大,但由于深色次生代谢产物在细胞腔的富集量不同,需结合含量测定及化学分析进一步判断。研究发现不同结香方法结香后木间韧皮部边缘1~2层薄壁细胞均无深色次生代谢产物沉积。打孔火烙法和两种不同程度剥皮法均刺激白木香形成再生组织,再生组织是否由IP分化而来还有待进一步研究;化学试剂A刺激未造成白木香形成再生组织且未见明显木质化,成功避免了阻隔层的形成,因此可形成块状结香区域;虫蛀样品可能因创伤时间较短而尚未形成再生组织,但沉香层木间韧皮部中的细胞明显木质化。新蛀虫口处胼胝质堆积,说明胼胝质参与了创伤防御反应。Agarwood is mainly obtained from Aquilaria sinensis(Lour.)Spreng,which is widely planted as precious tree species in subtropical areas of China.However,the development of the agarwood industry is significant restricted,mainly due to the low production efficiency and poor product quality caused by the current garwood induced technology.To learn the physiological process of induced agarwood in A.sinensis and help to develop artificial agarwood induced technology,anatomy of A.sinensis using several agarwood induced methods,which included the scorched holing method,bark peeling method,insect larvae gnawed and chemical reagent A induced method,were analyzed.It was found that the differences of formation and distribution were observed between different methods,including the dark secondary metabolites deposition,regenerated tissue formation,callose deposition,crystal distribution,etc.Among all methods,only chemical reagent A induced method resulted in blocky deposited area of dark secondary metabolites(agarwood formation),while other methods resulted in thin layered area,fasciculate area or punctate area.The comparison of agarwood formation areas showed that the chemical reagent A induced method>bark peeling method with xylem injured/scorched holing method>fresh insect larvae gnawed>bark peeling method without scathed xylem.It was preliminarily estimated that the chemical reagent A method resulted in largest agarwood formation area.However,due to the different deposition amount of dark secondary metabolites in the single cell cavity,it was needed to be further determined refer to the extraction of content determination and chemical analysis.It was indicated that resin did not deposit in the edge 1 or 2 cells of the interxylary phloem in different induced methods.The scorched holing method and two degree bark peeling method stimulated the dedifferentiation of A.sinensis to form regenerated tissue,it was worthy of being further studied that whether the interxylary phloem formed regenerated tissue;chemical reagent A method

关 键 词：白木香 结香方法 解剖构造 木间韧皮部 沉香 

分 类 号：S781.1[农业科学—木材科学与技术]