抑癌扶正平衡Ⅱ号方对乳腺癌小鼠移植瘤生长转移的实验研究  

Experimental research on growth and metastasis of transplanted tumor in mice with breast cancer treated with Yi’ai Fuzheng No.Ⅱ Recipe

作　　者：艾望 周珍[1] 王秀萍[1] 张曼玲 杨海涛 张莹雯[1] AI Wang;ZHOU Zhen;WANG Xiuping;ZHANG Manling;YANG Haitao;ZHANG Yingwen(Zhongnan Hospital of Wuhan University,Wuhan 430071;Hubei University of Traditional Chinese Medicine,Wuhan 430065,China)

机构地区：[1]武汉大学中南医院,武汉430071 [2]湖北中医药大学,武汉430065

出　　处：《天津中医药大学学报》2019年第4期378-382,共5页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：湖北省2016—2017年度卫计委中西医结合科研重点项目;湖北省自然科学基金青年项目(2015CFB640);湖北省自然科学基金项目(2009CHB001)

摘　　要：[目的]观察抑癌扶正平衡Ⅱ号方对乳腺癌小鼠移植瘤生长及肺转移的抑制作用,并探索其抗肿瘤效应与硫酸乙酰肝素蛋白聚糖的相关性。[方法]建立4T1乳腺癌小鼠模型,随机分为空白组、模型组、低分子肝素组、抑癌扶正平衡Ⅱ号方高、中、低剂量组,连续给药14 d后处死取材。测定移植瘤瘤质量和计数肺转移结节个数,并计算瘤质量抑制率及肺转移抑制率。ELISA法检测各组小鼠移植瘤、转移瘤、血清中硫酸乙酰肝素蛋白聚糖(HSPG)的表达。[结果]中药各组瘤质量、肺转移结节数随中药剂量增高而下降(P<0.05);高剂量中药组瘤质量抑制率及肺转移抑制率均优于低分子肝素组(P<0.05)。中药各组移植瘤、转移瘤、血清中HSPG的表达随中药剂量增高而上升(P<0.05);高剂量中药组移植瘤、转移瘤、血清中HSPG的表达高于低分子肝素组(P<0.05)。[结论]抑癌扶正平衡Ⅱ号方可显著抑制乳腺癌小鼠移植瘤的增殖和肺转移,其作用机制可能与增高硫酸乙酰肝素蛋白聚糖的表达有关。[Objective]To observe the inhibitory effect of Yi’ai Fuzheng No.II recipe on tumor growth and lung metastasis in breast cancer xenografts in mice,and to explore the relationship between its anti-tumor effect and heparan sulfate proteoglycan.[Methods]The mice model of 4T1 breast cancer was established and randomly divided into blank group,model group,low molecular weight heparin group,high-,medium-,and low-dose groups of Chinese medicine.After 14 days of continuous administration,the samples were sacrificed.The tumor mass and the number of lung metastatic nodules were measured,and the tumor mass inhibition rate and lung metastasis inhibition rate were calculated.The expression of HSPG in transplanted tumor,metastatic tumor and serum of mice in each group was detected by Elisa method.[Results]The tumor quality and the number of lung metastatic nodules of the Chinese medicine group decreased with the increase of dose(P<0.05).The tumor mass inhibition rate and lung metastasis inhibition rate of the high dose Chinese medicine group were better than those of the low molecular weight heparin group(P<0.05).The expression of HSPG in transplanted tumor,metastatic tumor and serum of the Chinese medicine group increased with the increase of dose(P<0.05).The expression of HSPG in transplanted tumor,metastatic tumor and serum of high dose Chinese medicine group was higher than that of low molecular weight heparin group(P<0.05).[Conclusion]The Yi’ai Fuzheng No.Ⅱrecipe can significantly inhibit the proliferation and lung metastasis of transplanted tumor in mice with breast cancer,and its mechanism may be related to the increased expression of heparin sulfate proteoglycan.

关 键 词：乳腺癌 抑癌扶正平衡Ⅱ号方 硫酸乙酰肝素蛋白聚糖 

分 类 号：R285.5[医药卫生—中药学]