酵母糖基化改造工程研究进展  

Advances in Yeast Glycosylation Alteration Engineering

作　　者：刘思阳 王秀然 王茗宇 李全亮 胡祥坤 卢天成 Liu Siyang;Wang Xiuran;Wang Mingyu;Li Quanliang;Hu Xiangkun;Lu Tiancheng(Bioreactor and Drug Development Ministry of Education Engineering Research Center,Jilin Agricultural University,Changchun,130118)

机构地区：[1]吉林农业大学,生物反应器与药物开发教育部工程研究中心

出　　处：《基因组学与应用生物学》2019年第8期3580-3585,共6页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31302062);吉林省自然基金项目(20180101260JC)共同资助

摘　　要：酵母真核表达系统是常用的安全性较高的外源蛋白表达系统。酵母细胞内存在翻译后糖基化修饰过程,对其糖基化修饰系统进行改造可用于生产人源糖蛋白。研究表明,可以通过基因工程手段消除酵母特有的内源糖基化反应、引入哺乳动物细胞表达系统中糖基化类型等方法对酵母糖基化路径进行改造。近年来许多研究通过对酵母菌株糖基化位点突变、基因缺失等方法对酵母糖基化系统进行改造,探究糖基化修饰对蛋白质功能的影响,这为利用酵母生产治疗性蛋白和新型糖基化疫苗提供了新的思路。本综述将对近年来酵母糖基化改造成果及研究进展进行综述。Yeast eukaryotic expression system is a commonly high-safety eukaryotic expression system. The post-translational glycosylation modification process is exesit In yeast cell, and its glycosylation modification system can be used to produce human glycoproteins after the transformationn. Research suggested that yeast glycosylation pathways could be modified by genetic engineering methods though eliminated yeast-specific endogenous glycosylation reactions and introduced the glycosylation patterns from mammalian cell expression systems. In recent years, many researches have conducted to modify yeast glycosylation systems by mutating glycosylation sites and deleting genes in yeast strains to explored the effects of glycosylation modifications on protein function. These provided new ideas for producing therapeutic proteins and new type of glycosylation vaccines by the yeast. This review will summarized the achievements and research progress of yeast glycosylation in recent years.

关 键 词：糖基化改造 酵母糖工程 人源糖基化 

分 类 号：TS2[轻工技术与工程—食品科学与工程]