郁金香种质资源遗传多样性的ISSR分析  

Genetic Diversity of Tulip Germplasm Resource Revealed by ISSR Markers

作　　者：巨秀婷 潘阿青 蒋福娟 唐楠[1,2] 侯志强 Ju Xiuting;Pan Aqing;Jiang Fujuan;Tang Nan;Hou Zhiqiang(Plateau Flower Research Center of Qinghai University,Xining,810016;Qinghai Province Garden Plant Research Key Laboratory,Xining,810016;Datong Forestry Investigation Team,Datong,810100)

机构地区：[1]青海大学高原花卉研究中心,西宁810016 [2]青海省园林植物研究重点实验室,西宁810016 [3]青海省大通县林业调查队,大通810100

出　　处：《基因组学与应用生物学》2019年第8期3667-3674,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31660582);2016年青海省园林植物研究重点实验室专项资金项目共同资助

摘　　要：本研究利用22个ISSR引物对5个新疆野生郁金香种和19个郁金香栽培品种进行ISSR分析。扩增得到195条谱带,其中多态位点为191个,多态性位点百分比为97.95%。24个郁金香材料检测等位基因数(na~*)平均为1.979 5,有效等位基因数(ne~*)在1.000 0~2.000 0之间,平均为1.432 3;Nei’s基因多样性指数(h~*)在0.000 0~0.500 0之间,平均为0.262;Shannon’s信息指数(I~*)在0.000 0~0.693 1之间,平均为0.407 8;研究实验反映出郁金香遗传变异水平较高,具有丰富的遗传多样性。聚类实验表明:24个郁金香材料在阈值为0.54处被聚类为A和B两大类,在阈值为0.63处除伊犁郁金香(T. iliensis)以外,其余四个郁金香野生种与常规栽培品种被单独聚类,说明野生种与栽培品种之间遗传距离较远,且大部分栽培品种之间亲缘关系较近。5个野生郁金香种中垂蕾郁金香(T. patens)、柔毛郁金香(T. buhseana)、天山郁金香(T. tianschanica)与栽培品种之间的遗传距离均在0.4以上,可以考虑作为杂交育种的材料。本研究为合理利用我国野生郁金香种质资源提供分子水平上的研究基础,并为日后郁金香杂交育种工作中亲本组合的选择提供一定的理论参考。In this study, 22 ISSR primers were used to analyze the genetic diversity of 5 wild species from Xinjiang province and 19 cultivars of tulip, which generated 195 bands that contains 191 polymorphic bands accounting for 97.95% of polymorphism rate. The tested 24 materials of tulips have an average of 1.979 5 of the number of alleles(na~*), the effective number of alleles(ne~*) ranges from 1.000 0~2.000 0 with the average of1.432 3;Nei’s genetic diversity index(h~*) ranges from 0.000 0 to 0.500 0 with average of 0.262;Shannon’s information index(I~*) ranges from 0.000 0 to 0.693 1 with an average of 0.407 8. These genetic parameters showed that the tested tulips have high genetic variations as well as rich genetic diversity. The clustering analysis showed that the tested 24 tulips except for T. iliensis in the threshold of 0.54 go into two categories, while the four wild species of tulips in the threshold of 0.63 are separated from the cluster of cultivated varieties. The genetic distance between wild species and cultivated varieties is quite far, whereas the genetic distance within the most of the cultivated varieties is close. Among the 5 wild tulips, the genetic distance among T. patens, T. buhseana, T.tianschanica and cultivated varieties is above 0.4,which can be considered as parental materials for breeding. This study provides a molecular basis fo r the rational utilization of wild tulip germplasm resources in China, and provides a theoretical reference for the selection of parent combinations in tulip hybridization in the future.

关 键 词：郁金香 种质资源 ISSR 遗传多样性 

分 类 号：S68[农业科学—观赏园艺]