矮牵牛冷响应转录因子PhZPT2-12的特性及表达分析  

Characterization and Expression Analysis of Petunia PhZPT2-12 Transcription Factor Related to Cold Response

作　　者：张慧琳 朱婉 田丽 张蔚[1] ZHANG Huilin;ZHU Wan;TIAN Li;ZHANG Wei(College of Horticulture&Forestry Sciences,Huazhong Agricultural University,Key Laboratory of Horticultural Plant Biology(Ministry of Education),Wuhan 430070,China)

机构地区：[1]华中农业大学园艺林学学院园艺植物生物学教育部重点实验室

出　　处：《园艺学报》2019年第8期1543-1552,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31772342);中国博士后科学基金项目(2016M592353)

摘　　要：从矮牵牛‘H’自交系中分离获得1个响应低温胁迫的C2H2型锌指蛋白基因PhZPT2-12,其开放阅读框为525 bp,编码1条含有两个典型的C2H2型保守结构域,长度为174 aa的氨基酸多肽链。系统进化树分析表明,PhZPT2-12与已报道的SlZF3亲缘关系较近。亚细胞定位和转录激活活性分析显示,PhZPT2-12定位于细胞核,是一种核蛋白,并且其全长在酵母细胞中不具有转录激活活性。半定量RT-PCR表达分析结果表明,正常生长条件下PhZPT2-12在矮牵牛根、茎、叶中表达量较高,而在成熟的花中仅有微弱表达;在低温、高盐和干旱胁迫处理下PhZPT2-12表现出不同程度的上调,其中对低温胁迫最为敏感,上调倍数最高,初步推测该基因与矮牵牛应答低温、高盐等非生物逆境相关。Gene expression microarray of petunia was applied to select cold responsive genes,from which a C2H2-type zinc-finger protein gene PhZPT2-12 was discovered.Using inbred line‘H’as the template,PhZPT2-12 was isolated.It contains an Open Reading Frame(ORF)of 525 bp,encoding a peptide of 174 aa with two classical C2H2 domains.Phylogenetic tree analysis demonstrated that PhZPT2-12 is closely related to SlZF3 reported.Subcellular localization and transactivation activity assays showed that PhZPT2-12 was targeted to nucleus and owned no transcriptional activation activity.Semi-quantitative RT-PCR analysis revealed that the expression of PhZPT2-12 was higher in roots,stems and leaves,whereas much lower in mature flowers under normal growth conditions.Stress induced expression analysis showed that the expression of PhZPT2-12 could be induced by cold rapidly,also by salt and drought in different degrees.It is suggested that PhZPT2-12 is associated with the responses to cold,salt and other abiotic stresses.

关 键 词：矮牵牛 低温胁迫 C2H2锌指蛋白 转录因子 表达分析 

分 类 号：S681.6[农业科学—观赏园艺]