雷公藤CYP450生物信息学及组织分布分析  

Bioinformatics and tissue distribution analysis of Tripterygium wilfordii CYP450

作　　者：高杰 张逸风[1,3] 周家伟 吴晓毅[1] 高伟 黄璐琦[2] GAO Jie;ZHANG Yi-feng;ZHOU Jia-wei;WU Xiao-yi;GAO Wei;HUANG Lu-qi(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences,Beijing 100700,China;School of Pharmaceutical Sciences,Capital Medical University,Beijing 100069,China;Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [3]首都医科大学药学院,北京100069 [4]首都医科大学人脑保护高精尖创新中心,北京100069

出　　处：《中国中药杂志》2019年第16期3594-3600,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81773830);北京市自然科学基金项目;北京市教育委员会科技发展计划重点项目(KZ201710025022);北京市属高校长城学者培养计划项目(CIT&TCD20170324toW.G.);“万人计划”青年拔尖人才项目

摘　　要：细胞色素P450家族是一种广泛存在于自然界中的生物催化剂,它具有催化植物次生代谢产物生物合成、调节植物修复等多种功能。该研究基于对雷公藤蛋白组数据的分析,利用多种生物信息学分析的方法对雷公藤CYP450基因进行了初步分析和预测,文章结果显示,使用茉莉酸甲酯诱导雷公藤悬浮细胞表达后,获取到雷公藤蛋白组数据并对其进行分析,最终筛选出10条雷公藤CYP450蛋白;通过分析与拟南芥家族CYP450基因构建得到的系统进化树,将这10条CYP450蛋白聚类归为6个不同的CYP450家族,各个家族CYP450蛋白质的理化性质存在一定差异,二级结构主要以不规则卷曲为主要构成元件,其中8条CYP450蛋白亚细胞定位结果为叶绿体,其余为质体;随后,通过对多重序列比对结果进行分析,找到了CYP450基因共有的保守结构域(亚铁血红素活性位点);最后,通过分析雷公藤转录组数据,初步确认了其在不同组织中的表达分布,从而验证了其与雷公藤活性物质生物合成具有相关性,为雷公藤有效活性成分生物合成途径解析提供了重要的基因资源。Cytochrome P450 family is a kind of biocatalyst widely existing in nature.It has many functions such as catalyzing the bi-osynthesis of plant secondary metabolites and regulating phytoremediation.Based on the analysis of proteome data of Tripterygium wilfor-dii,the CYP450 gene of T.wilfordii was preliminarily analyzed and predicted by various bioinformatics methods.The results showed that after the expression of T.wilfordii suspension cells was induced by methyl jasmonate,the proteomic data of T.wilfordii were ob-tained and analyzed,and 10 CYP450 proteins of T.wilfordii were finally screened out.By analyzing the phylogenetic tree constructed with CYP450 gene of Arabidopsis family,the 10 CYP450 proteins were clustered into 6 different CYP450 families.The physical and chemical properties of CYP450 proteins in different families were different.The secondary structure of CYP450 proteins was mainly composed of irregular curls.Eight subcellular localization results of CYP450 proteins were chloroplasts and the rest were plastids.Sub-sequently,the conserved domains(heme active sites)shared by CYP450 genes were found by analyzing the results of multiple sequence alignment.Finally,by analyzing the transcriptome data of T.wilfordii,the expression distribution of T.wilfordii in different tis-sues was preliminarily confirmed,which verified its correlation with the biosynthesis of active components of T.wilfordii,and provided important genetic resources for the analysis of biosynthesis pathway of active components of T.wilfordii.

关 键 词：雷公藤 CYP450 生物信息学 

分 类 号：R28[医药卫生—中药学]