新生大鼠高氧性肺损伤肺组织中AQP1、α-ENaC蛋白和mRNA表达及呋塞米雾化的干预作用  

The Expression of AQP1, α-ENaC Protein and mRNA and the Effect of Intravenous Frusemide on Acute Hyperoxia-Induced Lung Injury in Neonatal Rats

作　　者：余敏[1] 肖志辉[1] YU Min;XIAO Zhi-hui(Department of Neonatology,Children,s Hospital of Soochow University,Suzhou,Jiangsu,215003,China)

机构地区：[1]苏州大学附属儿童医院新生儿科,江苏苏州215003

出　　处：《中国血液流变学杂志》2019年第1期17-21,F0002,共6页Chinese Journal of Hemorheology

摘　　要：目的 探讨呋塞米雾化对新生大鼠高氧肺损伤的保护作用.方法 将新生SD大鼠随机分为空气组、高氧组和干预组,比较肺组织病理改变、肺组织AQP1,α-ENaC蛋白及mRNA表达情况.结果 空气组中的实验动物反应好,高氧组实验动物表现脱氧后烦躁不安,呼吸困难明显,其中1 只实验动物死亡.而干预组中的实验动物一般活动情况较高氧组明显改善.三组中高氧组肺组织形态与结构改变最为严重.在实验3、7、14 d时,高氧组肺组织AQP1蛋白及mRNA表达水平低于空气组(P<0.05),干预组肺组织AQP1蛋白及mRNA表达水平在实验3、7、14 d时均与空气组接近,明显高于高氧组,差异有统计学意义(P<0.05).在实验3、7、14 d时,高氧组肺组织α-ENaC的蛋白及mRNA表达水平较空气组明显下降(P<0.05),而干预组肺组织α-ENaC蛋白及mRNA表达水平较高氧组明显升高,其差异具有统计学意义(P<0.05).结论 雾化吸入呋塞米可能通过上调AQP1、α-ENaC的蛋白表达水平及mRNA表达水平,改善肺功能,从而对高氧性肺损伤起到一定保护作用.Objective To investigate whether furosemide inhalation could protect against hyperoxia-induced lung injury. Methods Neonatal rats were randomly assigned to three groups: control group, hyperoxia group and furosemide group, to compare the difference about lung histology and the protein and mRNA expression of AQP1 andα-ENaC. Results In control group, the growth of rats was normal with no death. In hyperoxia group, the experimental animals'spirit was dispirited, showing dyspnea and cyanotic without hyperoxia. The general condition of rats in furosemide group compared with hyperoxia group was improved. The morphological and structural changes of lung tissue were most severe in the three groups of hyperoxia. On the 3rd, 7th and 14th day of the experiment, the expression of AQP1 protein and mRNA in the lung tissue of the hyperoxia group was lower than that of the control group (P<0.05). The expression of AQP1 protein and mRNA in the lung tissue of the intervention group was 3 days. At 7 days and 14 days, they were close to the air group, which was significantly higher than that in the hyperoxia group, and the difference was statistically significant (P<0.05). At the 3rd, 7th and 14th day of the experiment, the expression of α-ENaC protein and mRNA in the hyperoxia group was significantly lower than that in the air group (P<0.05), while the expression of α-ENaC protein and mRNA in the lung tissue of the intervention group was observed. The hyperoxia group was significantly elevated, and the difference was statistically significant (P<0.05). Conclusion Inhalation of furosemide may improve the lung function by up-regulating the protein expression level and mRNA expression level of AQP1 and α-ENaC, thereby protecting the hyperoxia-induced lung injury.

关 键 词：急性高氧性肺损伤 新生大鼠 呋塞米 水通道蛋白 钠通道蛋白 

分 类 号：R722[医药卫生—儿科]