电活性和生物活性多巴-胰岛素样生长因子-1@聚(乙交酯-丙交酯)/聚(3-己基噻吩)静电纺丝纤维的制备及神经组织工程应用  

Preparation of Electroactive and Bioactive DOPA-Jointed Insulin-Like Growth Factor-1@Poly(glycolide-lactide)/Poly(3-hexylthiophene) Electrospinning Fiber and Its Application in Neural Tissue Engineering

作　　者：张守燕 胡江磊[1] 史新翠[2] 章培标[2] 伊藤嘉浩 ZHANG Shouyan;HU Jianglei;SHI Xincui;ZHANG Peibiao;ITO Yoshihiro(School of Chemical Engineering&Advanced Institute of Materials Science,Changchun University of Technology,Changchun 130012,China;Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022 China;Nano Medical Engineering Laboratory,RIKEN,Japan)

机构地区：[1]长春工业大学化学工程学院,长春130012 [2]中国科学院长春应用化学研究所,高分子生态材料重点实验室,长春130022 [3]日本理化学研究所,纳米医学工程实验室,日本

出　　处：《应用化学》2019年第9期1003-1014,共12页Chinese Journal of Applied Chemistry

基　　金：吉林省科技发展计划项目(20170520141JH)资助~~

摘　　要：理想型神经修复材料应具备与正常神经相似的导电性、仿生细胞外基质结构以及释放特定的生长因子等性能。本研究将不同质量分数(0、3%、5%、10%)的聚(3-己基噻吩)(P3HT)加入到聚(乙交酯-丙交酯)(PLGA)中,采用静电纺丝工艺,制备了具有电活性和仿生结构的复合纤维。利用酪氨酸羟化酶,将不同质量浓度(10、50、100 ng/mL)的含多巴接头的胰岛素样生长因子-1(DOPA-IGF-1)绑定在纤维表面,实现生长因子长效稳定的作用。通过扫描电子显微镜、接触角表征了纤维直径、分布以及表面亲疏水性。利用细胞培养、荧光染色实验评估了纤维在体外的生物相容性和生物活性。结果表明,该电活性纤维能有效促进大鼠肾上腺嗜铬细胞瘤细胞(PC12)增殖,其中,PLGA/P3HT-5%纤维表现出更好的细胞响应性。结合DOPA-IGF-1质量浓度为10 ng/mL的纤维更利于PC12细胞的黏附、生长。兼具电活性和生物活性的纳米纤维DOPA-IGF-1@PLGA/P3HT在神经组织修复领域具有潜在的应用价值。An ideal biomaterial for nerve repair should have the properties of similar electrical properties to normal nerve,biomimetic extracellular matrix structure and the release of specific growth factors.In this study,composite fibers with electroactive and bionic structures were prepared by electrospinning process by incorporating various mass fractions(0,3%,5%,10%)poly(3-hexylthiophene)(P3HT)into the poly(glycolide-lactide)(PLGA)polymer.DOPA-jointed insulin-like growth factor-1(DOPA-IGF-1)with different concentrations(10,50,100 ng/mL)was bound to the fiber surface using tyrosine hydroxylase to achieve long-term and stable release of growth factor.The fiber diameter and surface hydrophilic properties of the material were characterized by scanning electron microscopy and contact angle measurement,respectively.The biocompatibility and bioactivity of the fibers in vitro were evaluated by cell culture and fluorescence staining experiments.It was found that the electroactive fiber could effectively promote the proliferation of rat adrenal pheochromocytoma cells(PC12 cells),and PLGA/P3HT-5%fiber showed better cell responsiveness.Fibers incorporated with DOPA-IGF-1 with a concentration of 10 ng/mL were more conducive to PC12 cell adhesion and growth.The results showed that DOPA-IGF-1@PLGA/P3HT with both electrical and biological activity had potential application in neural tissue repair.

关 键 词：电活性 生长因子 聚噻吩 聚酯复合物 静电纺丝 神经组织工程 

分 类 号：O633[理学—高分子化学]