通络化痰胶囊对MCAO模型大鼠皮层Nogo-A、GAP-43表达影响的实验研究  

Effect of tongluo huatan capsules on the expressions of cortical Nogo-A and GAP-43 in MCAO model rats

作　　者：何嘉盛 苏春燕[1] 赖敏 范吉平[2] 周杰 HE Jia-sheng;SU Chun-yan;LAI Min;FAN Ji-ping;ZHOU Jie(Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102;China Academy of Chinese Medical Sciences,Beijing 100700;Department of Neurosurgery,Beijing First Integrated Traditional Chinese and Western Medical Hospital,Beijing 100018)

机构地区：[1]中国中医科学院望京医院,北京100102 [2]中国中医科学院,北京100700 [3]北京市第一中西医结合医院神经外科,北京100018

出　　处：《世界中西医结合杂志》2019年第8期1115-1118,共4页World Journal of Integrated Traditional and Western Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金资助(ZZ0708094)

摘　　要：目的研究通络化痰胶囊对MCAO模型大鼠脑缺血损伤后皮层区域神经轴索抑制因子勿动蛋白(Nogo-A)及轴突再生促进因子生长相关蛋白43(GAP-43)表达的影响。方法48只Wistar大鼠随机分为正常组、模型组、通络化痰胶囊高、低剂量组。除正常组外,其余各组参照Koizumi法制作大鼠大脑中动脉缺血模型(MCAO)。高、低剂量组造模清醒后分别给予648 mg/kg、324 mg/kg通络化痰胶囊2 ml。各组大鼠同期观察7 d、14 d。应用免疫组化法检测Nogo-A、GAP-43表达水平。结果与模型组相比,通络化痰胶囊高、低剂量组均能降低Nogo-A表达(P<0.05,P<0.01),上调GAP-43的表达(P<0.05)。结论通络化痰胶囊可通过降低Nogo-A的表达、上调GAP-43的表达,改善脑缺血区域神经轴突再生的微环境,从而发挥其脑保护作用。Objective To study the effect of tongluo huatan capsules on the expressions of Nogo-A and GAP-43 in the cortex after ischemia injury in MCAO model rats.Methods A total of 48 Wistar rats were randomized into a normal group,a model group,a high-dose group and a low-dose group of tongluo huatan capsules.In reference to Koizumi method,MCAO model was prepared in the rats.The IHC method was adopted to determine the expressions of Nogo-A and GAP-43.Results Compared with the model group,in the high-dose and the low-dose group of tongluo huatan capsules,Nogo-A expression was reduced(P<0.05,P<0.01)and GAP-43 was down-regulated(P<0.05).Conclusion Tongluo huatan capsules improve the microenvironment of axon regeneration in cerebral ischemic area,thus play the role to the brain protection.

关 键 词：通络化痰胶囊 神经轴索抑制因子勿动蛋白 轴突再生促进因子生长相关蛋白43 脑缺血 脑保护 

分 类 号：R285.5[医药卫生—中药学]