基于UPLC-Q-TOF/MS技术的血栓心脉宁片入血成分分析  

Analysis of Blood Components of Xueshuan Xinmaining Tablet Administered to Rats by Oral Administration

作　　者：谭静 林红强 刘云鹤 王涵 董庆海 吴福林 朱继忠[2] 刘传贵[2] 李平亚[1] 刘金平[1] 刘俊丽 TAN Jing;LIN Hong-qiang;LIU Yun-he;WANG Han;DONG Qing-hai;WU Fu-lin;ZHU Ji-zhong;LIU Chuan-gui;LI Ping-ya;LIU Jin-ping;LIU Jun-li(Natural Product Research Center,College of Pharmacy,Jilin University,Changchun 130021,China;Jilin Huakang Pharmaceutical Company Limited,Dunhua 133700,China)

机构地区：[1]吉林大学药学院天然药物研究中心,长春130021 [2]吉林华康药业股份有限公司,吉林敦化133700

出　　处：《特产研究》2019年第3期89-96,共8页Special Wild Economic Animal and Plant Research

基　　金：吉林省省校共建中药专项项目（SXGJSF2017-1-1）

摘　　要：基于血清药物化学方法,研究血栓心脉宁片(XXT)灌胃给予血瘀大鼠的血中移行成分。通过冰水浴-肾上腺素法复制急性血瘀大鼠模型,并灌胃给予XXT〔1.4 g/(kg·d)〕8 d,末次给药后收集血清样本,继而采用超高效液相色谱-飞行时间质谱联用(UPLCQ/TOF-MS)技术,结合多元统计分析,鉴定XXT的血中移行成分。从血瘀大鼠血清中鉴定出包括菲醌、三萜皂苷及甾体等结构类型的28个原型入血成分及3个代谢产物。UPLC-Q-TOF/MS分析方法的建立,对XXT的血中移行成分进行了全面阐释,为该中药大品种的药效物质基础研究奠定了基础。Based on the serum medicinal method,the blood-transition constituents in rats serum after oral administration of Xueshuan Xinmaining Tablet(XXT)were quickly and comprehensively analyzed and identified.The acute blood stasis rat model was replicated by ice bath-adrenalin method,and XXT〔1.4 g/kg·d〕was administered orally for 8 days.The serum samples were collected and pretreated after oral administration for the last time.Then,the absorbed prototype constituents and their metabolites in rats plasma were rapidly analyzed and identified by UPLC-Q-TOF/MS,an ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry,coupled with multivariate statistical analysis.Finally,a total of 28 absorbed prototype constituents and 3 metabolites were identified,including phenanthrene,triterpenoid saponins,steroids and other structural types of compounds.these constituents absorbed into blood may be the potential bioactive components in XXT.The establishment of UPLC-Q-TOF/MS analysis method explained comprehensively the blood migration component of XXT,and provided scientific basis for clarifying its substance basis of pharmacodynamics of this large Chinese medicine.

关 键 词：血栓心脉宁片 入血成分 UPLC-Q-TOF/MS 灌胃给药 

分 类 号：R927.2[医药卫生—药学]