湖羊IGF-1基因5’侧翼区多态性与羊毛弯曲度性状的关联分析  

Polymorphism of the 5’ Flanking Region of IGF-1 Gene in Hu Sheep and Its Association with Wool Curvature Traits

作　　者：李芝丰 周翰林 孙伟[1] 盛水兴 陈玲[2] Li Zhifeng;Zhou Hanlin;Sun Wei;Sheng Shuixing;Chen Lin(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009,China;Animal Science and Veterinary Medicine Bureau of Suzhou City,Suzhou,Jiangsu 215128,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省苏州市畜牧兽医站,苏州215128

出　　处：《中国草食动物科学》2019年第5期14-17,共4页China Herbivore Science

基　　金：国家自然科学基金(31872333);科技部家养动物平台项目;江苏省重点研发计划(现代农业)项目(BE2018354);江苏省农业重大新品种创制项目(PZCZ201739);江苏省农业科技自主创新项目(CX(18)2003);江苏高校优势学科建设工程资助项目

摘　　要：为探寻湖羊IGF-1基因5′侧翼区多态性及其对羊毛弯曲度性状的影响,选用128只湖羊作为实验材料,对IGF-1基因5'侧翼区进行扩增、测序和PCR-SSCP检测,探讨IGF-1基因5′侧翼区的遗传特征,寻找与羊毛弯曲度性状相关的遗传标记,为湖羊羊毛弯曲度标记辅助选择提供理论依据。结果表明:湖羊IGF-1基因5'侧翼区突变位点有AA、AB、BC三种基因型,基因型频率分别为0.609 4、0.093 8和0.296 8。AB型与AA型相比,在外显子1前的第649 bp有一处G→A突变,BC型与AA型相比,在外显子1前的第651 bp处发生G→C突变,在外显子1前的第649 bp有一处G→A突变。卡方独立性检验结果表明,不同基因型个体羊毛花纹类型和基因型之间不存在显著相关关系(P>0.05)。该研究初步认为,IGF-1基因5'侧翼区多态性与羊毛弯曲度性状之间没有显著关联(P>0.05)。The purpose of this study was to explore the polymorphism of the 5′flanking region of IGF-1 gene in Hu sheep and its effect on wool curvature traits.Amplification,sequencing and PCR-SSCP of the 5'flanking region of IGF-1 gene were performed using 128 Hu sheep as experimental materials.To explore the genetic characteristics of the 5'flanking region of IGF-1 gene,and to search for genetic markers related to wool curvature traits,it is expected to provide a basis for the marker assisted selection of Hu sheep wool curvature.The results showed that there were three genotypes(AA,AB,BC)in the 5'flanking region of IGF-1 gene,and the genotype frequencies were 0.609 4,0.093 8 and 0.296 8,respectively.Compared with the AA type,the AB type had a G→A mutation at the 649 bp before exon 1.Compared with the AA type,the BC type had a G→C mutation at the 651 bp before exon 1,and a G→A mutation at the 649 bp before exon 1.The results of chi-square independence test showed that there was no significant correlation between wool pattern and different genotypes(P>0.05).This study initially concluded that there was no significant association between the 5'flanking region polymorphism of the IGF-1 gene and the wool curvature trait(P>0.05).

关 键 词：湖羊 IGF-1基因 多态性 羊毛弯曲度 

分 类 号：S826.2[农业科学—畜牧学]