多溴联苯醚-47对淡水背角无齿蚌热休克蛋白基因表达的影响  

Effects of polybrominated diphenyl ether-47 on the Heat shock protein HSP60 expressions of freshwater bivalve Anodonta woodiana

作　　者：王涵 张珊[1] 薛士鹏[1] 宋国英 于瑞雪 刘庆春[1] 王中晓[1] 张庆远[1] 刘丽 李冰洁 夏西超[1,2] WANG Han;ZHANG Shan;XUE Shi-peng;SONG Guo-ying;YU Rui-xue;LIU Qing-chun;WANG Zhong-xiao;ZHANG Qing-yuan;LIU Li;LI Bing-jie;XIA Xi-chao(College of Basic Medicine,Nanyang Medical University,Nanyang 473061,Henan,China;College of Medicine,Pingdingshan University,Pingdingshan 476000,Henan,China)

机构地区：[1]南阳医学高等专科学校基础医学部,河南南阳473061 [2]平顶山学院医学院,河南平顶山476000

出　　处：《淡水渔业》2019年第5期3-9,共7页Freshwater Fisheries

基　　金：河南省高等学校重点科研项目(18A330004);河南省联合基金项目(182300410123);南阳市自然科学基础与前沿(2019JCQY010)

摘　　要：为了探讨多溴联苯醚-47(PBDE-47)对背角无齿蚌(Anodonta woodiana)慢性毒性效应,克隆了背角无齿蚌热休克蛋白基因(AwHSP60),分析基因全长、开放阅读框、编码、氨基酸。研究发现AwHSP60具有HSP60家族的标签序列;荧光定量PCR分析表达显示,AwHSP60基因广泛分布于斧足、鳃、肝胰脏、闭壳肌、心脏、血淋巴和外套膜,PBDE-47处理后肝胰脏中AwHSP60 mRNA水平在第1-15天显著性增加了89.9%,鳃中AwHSP60 mRNA水平显著性增加了2.09倍,血淋巴中AwHSP60 mRNA水平显著性增加2.13倍。结果表明,背角无齿蚌上调AwHSP60表达有助于增强动物对PBDE-47处理的耐受能力。In order to investigate the chronic effect of polybrominated diphenyl ether-47(PBDE-47),one heat shock protein 60 was cloned and named AwHSP60.Molecular characterizations of AwHSP60 were analyzed.AwHSP60 was widely distributed in foot,gill,hepatopancreas,adductor muscle,heart,hemocytes and mantle.Administration of PBDE-47 could result in a significant up-regulation of AwHSP60 expression in the hepatopancreas,gill and hemocytes.In the hepatopancreas,compared with that of control group,mRNA level of AwHSP60 increased more than 89.9%from day 1 to 15.In the gill,during experiment observed,expression of AwHSP60 increased more 2.09 times in contrasted with that of control group.In the hemocytes,AwHSP60 expression of PBDE-47 treated group increased more 2.09 times and 1.81 times compared with that of control group.These results indicated that up-regulation of AwHSP60 expression is contribute to enhancing adaption of bivalve A.Woodiana exposed to PBDE-47 treatment.

关 键 词：背角无齿蚌 ( ANODONTA woodiana ) 多溴联苯醚-47 ( PBDE-47) 背角无齿蚌热休克蛋白基因60(AwHSP60) 基因表达 

分 类 号：Q459[生物学—生理学]