干旱胁迫下种子引发对板蓝根种子萌发及幼苗生长的影响  

Effects of Seed Priming on Germination and Seedling Growth of Radix Isatidis under Drought Stress

作　　者：孟衡玲[1] 卢丙越 苏一兰[1] 王田涛 张薇[1] MENG Hengling;LU Bingyue;SU Yilan;WANG Tiantao;ZHANG Wei(Honghe University/The Key Laboratory of High Quality Crops Cultivation and Safety Control of Yunnan Province,Honghe 661100,China)

机构地区：[1]红河学院/云南省高校农作物优质高效栽培与安全控制重点实验室

出　　处：《河南农业科学》2019年第9期54-59,共6页Journal of Henan Agricultural Sciences

基　　金：云南省中青年学术带头人后备人才项目(2016PY068);红河学院学术带头人后备人才资助项目(2015HB0303)

摘　　要：为筛选适宜的板蓝根种子引发剂,采用PEG-6000模拟干旱胁迫,以PEG-6000、CaCl2、GA3、水4种不同引发剂对板蓝根种子进行引发处理,探讨板蓝根种子在干旱胁迫下的发芽特性及幼苗的生长状况。结果表明,干旱胁迫(20%PEG-6000模拟干旱)下,4种引发剂均能不同程度地提高板蓝根种子的发芽率、发芽势和发芽指数。综合比较来看,18 h水引发处理效果最好,种子发芽率、发芽势、发芽指数较CK2(干旱胁迫但未进行引发)分别提高33.3%、36.6%、106.4%。其次是25%PEG-6000引发处理,种子发芽率、发芽势、发芽指数较CK2分别提高35.2%、51.2%、32.8%;50 mg/L GA3引发处理下的种子发芽指数显著高于其他处理,较CK2提高171.1%,可有效提高种子的发芽速率;1.5%CaCl2处理引发效果最差,种子发芽率、发芽势和发芽指数较CK2分别提高16.7%、19.5%和23.9%。种子经过引发后,可不同程度提高板蓝根幼苗的株高、根长、叶绿素含量,降低叶片的相对电导率。综上,4种不同引发剂对板蓝根种子的引发效果依次为18 h水>25%PEG-6000>50 mg/L GA3>1.5%CaCl2,建议在板蓝根种子播种前进行18 h水引发处理。In order to screen out the appropriate seed initiator for Radix Isatidis,four initiators including PEG-6000,CaCl2,GA3 and H2O were used to treat the Radix Isatidis seeds,and then the seed germination and seedling growth characteristics of Radix Isatidis were investigated under 20%PEG-6000 simulated drought stress.The results showed that all these four initiators could improve seed germination rate,germination potential and germination index in different degrees under drought stress.The 18 h water priming had the best effect on seed germination,and the seed germination rate,germination potential,and germination index were increased by 33.3%,36.6%and 106.4%respectively compared with CK2(drought stress and no initiators).The follower was 25%PEG-6000 priming,and the germination rate,germination potential,germination index were 35.2%,51.2%,and 32.8%higher than CK2,respectively.The 50 mg/L GA3 priming had a significantly higher effect than other treatments on germination index,which was increased by 171.1%compared with CK2.The 1.5%CaCl2 had lowest effect on germination rate,germination potential and germination index,which only were increased by 16.7%,19.5%and 23.9%respectively compared with CK2.In addition,after seed priming,the plant height,root length,chlorophyll content of seedlings were significantly increased,and the relative conductivity was decreased.In conclusion,the seed priming effect was in turns:18 h water>25%PEG-6000>50 mg/L GA3>1.5%CaCl2,which suggested that 18 h water priming to treat the seeds before sowing could greatly improve the seed germination and seedling growth of Radix Isatidis.

关 键 词：板蓝根 种子引发 干旱胁迫 渗调引发 激素引发 水引发 种子萌发 

分 类 号：S567[农业科学—中草药栽培]