鹌鹑ESR1基因外显子1多态性与早期生长性能的关联分析  

Association between Polymorphism of Exon 1 of ESR1 Gene and Early Growth Performance in Quail

作　　者：曹恒 杨帅[1] 白俊艳[1] 樊红灯 庞有志[1] 付学言 卢小宁 时坤鹏 CAO Heng;YANG Shuai;BAI Junyan;FAN Hongdeng;PANG Youzhi;FU Xueyan;LU Xiaoning;SHI Kunpeng(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,China)

机构地区：[1]河南科技大学动物科技学院

出　　处：《河南农业科学》2019年第9期157-161,共5页Journal of Henan Agricultural Sciences

基　　金：河南科技大学大学生研究训练计划(SRTP)项目(2012251);河南省产学研合作项目(152107000095.0)

摘　　要：为了探讨雌激素受体基因1(ESR1)的多态性对鹌鹑早期生长性能的影响,采用PCR-RFLP法,对3个鹌鹑群体(中国黄羽鹌鹑、朝鲜鹌鹑、北京白羽鹌鹑)的ESR1基因外显子1进行多态性检测,并与鹌鹑早期生长性能进行关联分析。结果表明,ESR1基因外显子1在3个鹌鹑群体中均检测到3种基因型,分别为TT、CC、CT基因型,ESR1基因外显子1在中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑中的CC基因型频率分别为0.515、0.600、0.723,TT基因型频率分别为0.088、0.014、0.015,CT基因型频率分别为0.397、0.386、0.262。关联分析表明,ESR1基因外显子1与朝鲜鹌鹑的胫长、胸骨长、体长、胫围有相关性(P<0.05),与中国黄羽鹌鹑的体质量、胫长、胸深、体长、胸骨长有相关性(P<0.05),与北京白羽鹌鹑的体质量有相关性(P<0.05)。以上结果提示,ESR1基因外显子1可作为候选基因应用于蛋用鹌鹑早期生长性能的分子标记辅助选择。In order to investigate the effect of polymorphism of estrogen receptor gene 1(ESR1)on early growth performance of quails,three quail populations(Chinese yellow quail,Korean quail and Beijing white quail)were tested by PCR-RFLP,and the association between ESR1 gene exon1 and early growth performance of quails was analyzed.The results showed that three genotypes TT,CC and CT were detected in exon1 of ESR1 gene in three quail populations.The CC genotype frequencies of exon1 of ESR1 gene were 0.515,0.600 and 0.723,TT genotype frequencies were 0.088,0.014 and 0.015,and CT genotype frequencies were 0.397,0.386 and 0.262 respectively in Chinese yellow quails,Beijing white quails and Korean quails.The correlation analysis showed that exon1 of ESR1 gene was correlated with tibia length,sternum length,body length and tibia circumference of Korean quails(P<0.05).Exon1 of ESR1 gene was correlated with body weight,tibia length,chest depth,body length and sternum length of Chinese yellow quail(P<0.05).Exon1 of ESR1 gene was also correlated with body mass of Beijing white quail(P<0.05).These results suggest that exon1 of ESR1 gene can be used as a candidate gene for marker-assisted selection of early growth performance of egg quails.

关 键 词：鹌鹑 雌激素受体基因1(ESR 1) 早期生长性能 PCR-RFLP 

分 类 号：S839[农业科学—畜牧学]