袋鼠皮多肽对H2O2诱导人肝细胞LO2氧化损伤的影响  

Effect of kangaroo skin peptide on the oxidative damage of human hepatocyte LO2 cells induced by H2O2

作　　者：王碧雪 祝雅雯 何庆 赵晶华 陈清西[1] WANG Bixue;ZHU Yawen;HE Qing;ZHAO Jinghua;CHEN Qingxi(School of Life Sciences,Xiamen University,Xiamen 361102,China;Fujian Bosheng Biological Technology Co.,Ltd.,Fuzhou 350004,China)

机构地区：[1]厦门大学生命科学学院,福建厦门361102 [2]福建省博胜生物科技有限公司,福建福州350004

出　　处：《厦门大学学报（自然科学版）》2019年第5期701-706,共6页Journal of Xiamen University：Natural Science

基　　金：厦门大学横向课题(XDHT2017407A);福建省博胜生物科技有限公司项目

摘　　要：为探索袋鼠皮多肽如何修复受H2O2损伤的人肝细胞LO2,通过建立H2O2诱导LO2细胞氧化损伤模型,研究袋鼠皮多肽对氧化应激损伤的LO2细胞的修复作用.结果表明:袋鼠皮多肽在0~0.5μg/μL质量浓度范围内不仅对LO2细胞活性没有抑制作用而且显著提高了受损细胞的存活率,改善了细胞的形态;与此同时,显著降低了H2O2诱导的丙二醛和蛋白质羰基含量,减少了活性氧的累积.综上结果可见袋鼠皮多肽能减小细胞的氧化损伤,有望用于新的功能性食品和护肤产品.To assess the recovery effect of active kangaroo skin peptide(KSP),an oxidative damage model of human hepatocyte LO2 cells induced by H2O2 was established.It revealed that KSP with the mass concentration of 0 to 0.5μg/μL not only had no inhibition on viability of LO2 cells,but also significantly elevated cell viability and improved cell morphology by H2O2 damage.Besides,it significantly reduced the content of malondialdehyde(MDA)and protein carbonyl group induced by H2O2.Furthermore,we confirmed that KSP could reduce the accumulation of H2O2-induced reactive oxygen species(ROS).Taken together,our data show that KSP is able to reduce oxidative damage on cells and can be applied in novel functional food and skin care product.

关 键 词：袋鼠皮多肽 人肝细胞 氧化损伤 

分 类 号：Q516[生物学—生物化学]