冻干地黄提取物对糖尿病小鼠降糖作用的研究  

Hypoglycemic effect of freeze-dried Rehmannia extractsin diabetic mice

作　　者：刘贵昌[1] 谭丽媛 翟康欣 李敏 张淑蓉 Liu Guichang;Tan Liyuan;Zhai Kangxin;Li Min;Zhang Shurong(Department of Pharmacy,Shanxi Children′s Hospital,Taiyuan 030013,China)

机构地区：[1]山西省儿童医院药剂科,太原030013 [2]山西中医药大学中药学院

出　　处：《中国药物与临床》2019年第17期2908-2911,共4页Chinese Remedies & Clinics

基　　金：山西中医药大学科技创新团队项目(2018TD-008);山西省中药材中药饮片地方标准研究—地黄(冻干地黄)(2011028A);晋药综合开发利用协同创新中心项目(2017-JYXT-04)。

摘　　要：目的研究冻干地黄降糖的有效部位。方法冻干地黄75%的乙醇提取物分别用石油醚、乙酸乙酯、正丁醇萃取,得正丁醇、水部位,并将75%提取后的冻干地黄再以蒸馏水提取得水提物,各部位均进行四氧嘧啶致糖尿病小鼠实验,从而确定地黄降糖有效部位。结果与模型组相比,正丁醇部位对四氧嘧啶致糖尿病小鼠饮食量、饮水量、体质量方面有较好的改善作用,并且正丁醇部位治疗组能够显著降低糖尿病小鼠血糖水平。结论冻干地黄正丁醇提取部位有良好的降糖作用。Objective To determine the effective parts of freeze-dried Rehmannia for hypoglycemic purposes.Methods Seventy-five percent ethanol extracts were collected from freeze-dried Rehmannia by petroleum ether,ethyl acetate and n-butanol,and the n-butanol and soluble parts were taken,respectively.Distilled water was used to obtain aqueous extracts from the 75%ethanol extracts of freeze-dried Rehmannia.The effects of different parts of freeze-dried Rehmannia extracts in alloxan-induced diabetic mice were determined.The food intake,water intake,body weight,fasting blood glucose and glucose tolerance in the mice model were comprehensively evaluated,so as to determine the effective part of Rehmannia for hypoglycemic purposes.Results Compared with the model group,the n-butanol part significantly improved the food intake,water intake and body weight in alloxan-induced diabetic mice.Also,then-butanol part significantly reduced blood glucose levels in diabetic mice.Conclusion The freeze-dried Rehmannia n-butanol extraction part shows a good hypoglycemic effect.

关 键 词：冻干地黄 部位 降糖作用 

分 类 号：R28[医药卫生—中药学]