Coaxial nozzle-assisted electrohydrodynamic printing for microscale 3D cell-laden constructs  

作　　者：Hongtao Liang Jiankang He Jinke Chang Bing Zhang Dichen Li 

机构地区：[1]State key laboratory for manufacturing systems engineering,Xi’an Jiaotong University,Xi’an,China

出　　处：《International Journal of Bioprinting》2018年第1期27-34,共8页国际生物打印期刊

基　　金：This work was supported by the National Natural Science Foundation of China(51422508,51675412);Shaanxi Key Research and Development Program(2017ZDXMGY-058);the Fundamental Research Funds for the Central Universities.

摘　　要：Cell printing has found wide applications in biomedical fields due to its unique capability in fabricating living tissue constructs with precise control over cell arrangements.However,it is still challenging to print cell-laden 3D structures simultaneously with high resolution and high cell viability.Here a coaxial nozzle-assisted electrohydrodynamic cell printing strategy was developed to fabricate living 3D cell-laden constructs.Critical process parameters such as feeding rate and stage moving speed were evaluated to achieve smaller hydrogel filaments.The effect of CaCl2 feeding rate on the printing of 3D alginate hydrogel constructs was also investigated.The results indicated that the presented strategy can print 3D hydrogel structures with relatively uniform filament dimension(about 80μm)and cell distribution.The viability of the encapsulated cells was over 90%.We envision that the coaxial nozzle-assisted electrohydrodynamic printing will become a promising cell printing strategy to advance biomedical innovations.

关 键 词：ELECTROHYDRODYNAMIC PRINTING CELL PRINTING BIOPRINTING BIOFABRICATION tissue engineering 

分 类 号：TG6[金属学及工艺—金属切削加工及机床]