COX-2抑制剂对慢性前列腺炎大鼠射精时间的影响  

Effect of COX-2 inhibitor on ejaculation time of chronic prostatitis rats

作　　者：郑涛[1] 王瑞[1] 张天标[1] 吕坤龙 贾东辉[1] 杨帆[1] 张卫星[1] ZHENG Tao;WANG Rui;ZHANG Tianbiao;LYU Kunlong;JIA Donghui;YANG Fan;ZHANG Weixing(Department of Urology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院泌尿外科

出　　处：《郑州大学学报（医学版）》2019年第5期745-749,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关计划项目(172102310021)

摘　　要：目的:探讨COX-2抑制剂对前列腺炎(CP)大鼠射精时间的影响及其机制。方法:雄性Wistar大鼠随机分为模型组(n=12)、正常对照组(n=10)和COX-2抑制剂组(n=10)。模型组和COX-2抑制剂组大鼠自身免疫法建立CP模型。造模成功后,模型组大鼠18 mg/(kg·d)灌胃塞来昔布,正常对照组和COX-2抑制剂组给予生理盐水。连用4周后,进行交配实验,记录骑跨潜伏期(ML)、插入潜伏期(IL)及射精潜伏期(EL)。各组大鼠行前列腺组织HE染色;ELISA法检测血清TNF-α、IL-1β水平;免疫组化和Western blot法检测T13~L2和L5~S2段脊髓5-羟色胺(5-HT)、5-HT1A、5-HT2C及5-HT转运(5-HTT)蛋白的表达。结果:模型组大鼠表现出典型的前列腺炎症。与正常对照组比较,模型组大鼠EL缩短,血清IL-1β和TNF-α升高,T13~L2及L5~S2脊髓5-HT水平下降、5-HT1A水平升高,L5~S2脊髓5-HTT升高(P<0.05)。而COX-2抑制剂组前列腺炎症轻微,血清TNF-α、IL-1β水平、脊髓5-HT及其受体水平、EL均较模型组改善(P<0.05)。结论:CP大鼠EL缩短与前列腺炎症有关。COX-2抑制剂可以延长CP大鼠EL。Aim:o investigate the effect of COX-2 inhibitor on ejaculation time of rats with chronic prostatitis(CP)and its mechanism.Methods:Male Wistar rats were randomly divided into model group(n=12),normal control group(n=10),and COX-2 inhibitor group(n=10).CP rat models were established by autoimmune method.After 4 weeks for modeling,18 mg/(kg·d)celecoxib were administered by gavage in the model group,and physiological saline were used in the normal control and COX-2 inhibitor groups.After that,a mating experiment was performed to record the riding latency(ML),insertion latency(IL),and ejaculation latency(EL).HE of prostate tissue was performed.Serum TNF-αand IL-1βwere detected by ELISA.Immunohistochemistry and Western blot were used to detect 5-hydroxytryptamine(5-HT),5-HT1A,5-HT2C and 5-HT transporter(5-HTT)in the spinal cord of T13~L2 and L5~S2.Results:Prostatic inflammation were showed in the model group.Compared with the normal group,EL was shortened,serum IL-1βand TNF-αwere increased,and 5-HT levels in T13~L2 and L5~S2 spinal cord were decreased.The level of 5-HT1A was elevated,and the 5-HTT of L5~S2 spinal cord was elevated(P<0.05).In the COX-2 inhibitor group,prostate inflammation was mild,cytokine levels,spinal cord 5-HT and its receptor levels,and EL were improved compared with the normal control group(P<0.05).Conclusion:EL shortening in CP rats is associated with prostatic inflammation and pelvic pain.COX-2 inhibitors can prolong rat EL.

关 键 词：慢性前列腺炎 早泄 COX-2抑制剂 5-羟色胺 

分 类 号：R697[医药卫生—泌尿科学]