低强度脉冲激光治疗糖尿病大鼠神经病理性疼痛的实验研究  

Experimental study for treatment of low-intensity laser on neuropathic pain in diabetic rats

作　　者：陈波 鲍红 CHEN Bo;BAO Hong(Department of Anesthesiology,Xidian Group Hospital of Xi'an,Xi'an710077,Shaanxi,China)

机构地区：[1]西安市西电集团医院麻醉科

出　　处：《海南医学院学报》2019年第18期1365-1370,共6页Journal of Hainan Medical University

基　　金：陕西省自然科学基金项目(2018JQ8056)~~

摘　　要：目的:系统阐明830 nm波长的低强度脉冲激光对于神经病理性疼痛的治疗效果。方法:30只雄性SPF级Wistar大鼠平均等分至空白对照组、糖尿病周围神经病理性疼痛(DNP)组、糖尿病周围神经病理性疼痛协同激光治疗(DNP+Laser)组。对DNP组和DNP+Laser组通过腹腔注射链脲佐菌素(STZ)构建糖尿病模型,DNP+Laser组施加激光干预(700 Hz、60 mW),每天刺激3次,每次50 s,连续刺激8周。分别于STZ注射的第0、2、4、6和8周对各组实验动物的足底机械痛阈值和热痛阈值进行检测,并通过植入电极测量大鼠的感觉神经和运动神经传导速率。结果:DNP+Laser组足底机械痛阈值和热痛阈值在STZ注射的第4、6、8周显著高于DNP组(P<0.05),且DNP+Laser组感觉神经传导速率和运动神经传导速率在第4、6、8周也显著高于DNP组(P<0.05)。结论:低强度激光对于延缓糖尿病大鼠神经病理性疼痛的发病进程具有积极的作用效果,提示低强度脉冲激光疗法有望成为临床糖尿病神经病理疼痛治疗的一种有效的物理因子作用方案。Objective:To systematically identify the therapeutic effects of low-intensity laser with 830 nm on diabetic neuropathic pain.Methods:Thirty male SPF Wistar rats were equally assigned to the blank control(Control)group,diabetic neuropathic pain(DNP)group and diabetic neuropathic pain exposed to the laser treatment(DNP+Laser)group.The rats in the DNP group and DNP+Laser group were subjected to intraperitoneal injection with streptozotocin(STZ)to establish the diabetic animal model.The rats in the DNP+Laser group were exposed to laser stimulation(700 Hz,60 mW)with 3 sessions per day and 50 s per session for overall 8 weeks.The paw mechanical pain threshold levels and thermal pain threshold levels were measured at 0,2,4,6 and 8 weeks post the STZ administration,and the motor nerve conduction velocity and sensory nerve conduction velocity were also determined.Results:The rats in the DNP+Laser group showed significantly higher paw mechanical pain threshold levels and thermal pain threshold levels than the rats in the DNP group(P<0.05).Moreover,the motor nerve conduction velocity and sensory nerve conduction velocity in the DNP+Laser group were also significantly higher than those in the DNP group(P<0.05).Conclusion:Low-intensity laser stimulation has positive effects on the attenuation of the progression of neuropathic pain in diabetic rats,implying that low-intensity laser treatment has the potential to be an effective biophysical approach for diabetic neuropathic pain in clinics.

关 键 词：低强度激光 糖尿病神经病理性疼痛 热痛阈值 机械痛阈值 神经传导速率 

分 类 号：R587.2[医药卫生—内分泌]